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目的:建立脉舒胶囊中总黄酮的含量测定方法并确定其限度。方法:采用比色法,以芦丁为对照品,以亚硝酸钠-硝酸铝显色后,在510nm波长处测定吸收度,计算总黄酮含量。结果:芦丁在0.0108～0.0540mg·mL~(-1)浓度范围内与吸收度呈良好线性关系(r=0.9998);平均加样回收率为99.68%,RSD=1.13%。脉舒胶囊的总黄酮含量在27.9～30.2mg·粒~(-1)之间,其限度确定为以芦丁计不得少于26.0mg·粒~(-1)胶囊。结论:本方法简便、准确、稳定,可用于脉舒胶囊的质量控制。
Objective: To establish a method for determination of total flavonoids in Maishu Capsule and determine its limits. Methods: The colorimetric method was used to determine rutin as a reference substance. After sodium nitrite-aluminum nitrate coloration, the absorbance was measured at a wavelength of 510 nm to calculate the content of total flavonoids. Results: There was a good linear relationship between the concentration of rutin and the absorbance in the concentration range of 0.0108-0.0540 mg·mL -1 (r=0.9998). The average recovery rate of rutin was 99.68% and RSD was 1.13%. The total flavonoid content of Maishu capsules was between 27.9 and 30.2 mg·granules(-1), and the limit was determined to be not less than 26.0 mg·capsule(-1) capsules based on rutin. Conclusion: This method is simple, accurate and stable and can be used for the quality control of Maishu Capsule.