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目的 探讨干扰素α的疗效是否与调节Th1/Th2细胞因子的表达有关 ,并以此作为预测干扰素α疗效指标的可能性。方法 采用双抗体夹心法和逆转录PCR方法 ,对应用干扰素治疗的2 3例慢性乙型肝炎患者外周血淋巴细胞中Th1/Th2细胞因子的表达水平进行动态检测。结果 与正常对照组比较 ,慢性乙型肝炎患者外周血淋巴细胞培养上清液中的干扰素α(IFN γ)水平较低 ,但差异无显著意义 ;白细胞介素 4(IL 4)水平则升高 ,差异有显著意义。其外周血淋巴细胞中的mRNA表达水平亦如此。与治疗前比较 ,干扰素α治疗后 3和 6个月 ,在完全应答的患者中 ,IL 4水平下降 ;而IFN γ水平在 6个月时升高 ,差异均有显著意义。结论 慢性乙型肝炎患者的外周血淋巴细胞中Th1/Th2细胞因子的表达水平与干扰素α的疗效密切相关。检测Th1/Th2细胞因子的变化 ,可预测患者对干扰素α治疗的反应 ,对及时判断干扰素α疗效有一定作用。
Objective To investigate whether the therapeutic effect of interferon α is related to the regulation of Th1 / Th2 cytokines and to predict the efficacy of interferon α. Methods Th1 / Th2 cytokine expression in peripheral blood lymphocytes of 23 patients with chronic hepatitis B treated with interferon was detected by double antibody sandwich method and reverse transcription PCR. Results Compared with the normal control group, the level of IFN-γ in the supernatant of peripheral blood lymphocytes was lower in patients with chronic hepatitis B, but the difference was not significant. The level of IL-4 was increased High, the difference is significant. The same is true for the level of mRNA expression in peripheral blood lymphocytes. Compared with those before treatment, levels of IL-4 decreased in patients with complete response at 3 and 6 months after interferon alpha treatment, while levels of IFN-gamma increased at 6 months, with significant differences. Conclusion The expression of Th1 / Th2 cytokines in peripheral blood lymphocytes of patients with chronic hepatitis B is closely related to the efficacy of interferon α. Detecting changes in Th1 / Th2 cytokines can predict the patient’s response to interferon-α therapy, to determine the efficacy of interferon-α in a timely manner.