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目的:研究杜仲黄酮类化合物紫云英苷和黄芩素对成骨细胞特异性转录因子Osterix及破骨细胞抑制因子与核因子κB受体活化因子配体比值(OPG/RANKL)的影响。方法:采用MC3T3-E1 Subclone 14成骨细胞,不同浓度紫云英苷和黄芩素和维生素D3组加入质量浓度为30 mg·L-1作用细胞24 h后,用MTT法检测细胞增殖情况,成骨细胞接种于24孔板后,空白组加入培养基,给药组分别加入质量浓度为80 mg·L-1(高浓度),40 mg·L-1(中浓度),7.5 mg·L-1(低浓度)的含紫云英苷或黄芩素,作用24 h后,ELISA法检测钙离子活性,蛋白免疫印迹法检测Osterix,OPG以及RANKL的蛋白表达水平。结果:与空白组相比,紫云英苷和黄芩素可后明显促进MC3T3-E1 Subclone 14成骨细胞的增殖(P<0.05);明显上调OPG和Osterix的表达(P<0.05),同时能降低RANKL的表达(P<0.05)。结论:杜仲黄酮类化合物紫云英苷和黄芩素能促进MC3T3-E1Subclone 14成骨细胞增殖,并上调Osterix和OPG/RANKL。
OBJECTIVE: To study the effects of Boschniakia glycosides and baicalein, a flavonoid compound, on the ratio of osteoblast-specific transcription factor Osterix and osteoclast inhibitor to ligand of activator of nuclear factor κB (OPG / RANKL). Methods: MC3T3-E1 Subclone 14 osteoblasts were treated with different concentrations of alisin and baicalein and vitamin D3 for 24 h, and the cell proliferation was measured by MTT assay The osteoblasts were seeded on a 24-well plate, and the blank group was added to the medium. The drug group was given 80 mg · L-1 (high concentration), 40 mg · L-1 (medium concentration), 7.5 mg · L- 1 (low concentration) containing albin or baicalein, after 24 h, the activity of calcium ion was detected by ELISA and the protein expression of Osterix, OPG and RANKL was detected by Western blot. Results: Compared with the blank group, albin and baicalein significantly promoted the proliferation of MC3T3-E1 subclone 14 osteoblasts (P <0.05), and significantly up-regulated the expression of OPG and Osterix (P <0.05) Reduce RANKL expression (P <0.05). Conclusion: Eucommia ulmoides flavonoids glycosides and baicalein can promote MC3T3-E1Subclone 14 osteoblast proliferation, and up-regulate Osterix and OPG / RANKL.