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分别将含免疫刺激ＤＮＡ序列（ＩＳＳ）的质粒ｐＵＣ１９、ｐｃＤＮＡ３及编码ＩＬ２的真核表达质粒ｐｃＤＮＡ３ＩＬ２，与乙肝病毒ＤＮＡ疫苗共同注射于小鼠胫前肌内。定量检测免疫小鼠体内ＨＢｓＡｇ的表达及血清中抗ＨＢｓ的水平。结果显示：ｐＵＣ１９、ｐｃＤＮＡ３及ｐｃＤＮＡ３ＩＬ２对乙肝病毒ＤＮＡ疫苗在肌肉内表达ＨＢｓＡｇ无影响或有抑制，但它们均能显著促进疫苗诱导抗体的产生（Ｐ＜００１），抗体水平的增加与ＩＳＳ的数量呈剂量反应关系。
Plasmids pUC19, pcDNA3 and IL-2 encoding eukaryotic expression plasmid pcDNA3-IL-2 containing immune stimulating DNA sequence (ISS) were co-injected into the anterior tibial muscle of mice with hepatitis B virus DNA vaccine respectively. The expression of HBsAg in the immunized mice and the level of anti-HBs in serum were quantitatively detected. The results showed that: pUC19, pcDNA3 and IL-2 on hepatitis B virus DNA vaccine expression of HBsAg in muscle had no effect or inhibition, but they can significantly promote vaccine-induced antibody production (P <0 01), antibody levels Increasing the number of ISS dose-response relationship.