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用CO2激光(20.1 mW/mm2)辐照小麦种子3 min,待幼苗长至二叶一心时,用质量浓度为100 g/L的聚乙二醇6000(PEG6000)胁迫其幼苗,并通过添加过氧化氢酶(catalase,CAT;H2O2清除剂)、抗坏血酸(ascorbate,AsA;H2O2清除剂)和二苯基碘(diphenylene iodonium,DPI;NADPH氧化酶抑制剂),研究过氧化氢在CO2激光预处理提高小麦耐旱性中的作用。结果表明,激光预处理可使干旱胁迫的小麦幼苗丙二醛(malondialdehyde,MDA)含量显著降低(p<0.05),而超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)和CAT活性、叶绿素a、叶绿素b、类胡萝卜素含量和株高、根长和根干重却显著增加(p<0.05)。而经过适当剂量激光辐照干旱胁迫小麦幼苗再加外源CAT(L+P+C),AsA(L+P+A)或DPI(L+P+D)处理能有效逆转激光对干旱胁迫小麦幼苗的防护作用。从而说明激光预处理主要通过诱导内源H2O2产生而对植物在生理生化水平上起到一定的防护作用,且H2O2的形成与NADPH氧化酶途径有关。
Wheat seeds were irradiated with CO2 laser (20.1 mW / mm2) for 3 min. Seedlings were challenged with PEG6000 at a concentration of 100 g / L until seedlings grew as long as two leaves. The effects of hydrogen peroxide on CO2 laser pretreatment (catalase, CAT; H2O2 scavenger), ascorbate (AsA; H2O2 scavenger) and diphenylene iodonium (DPI; NADPH oxidase inhibitor) Improve wheat drought tolerance role. The results showed that laser pretreatment could significantly reduce malondialdehyde (MDA) content of wheat seedlings under drought stress (p <0.05), but superoxide dismutase (SOD), peroxidase POD and CAT activity, chlorophyll a, chlorophyll b, carotenoid content and plant height, root length and root dry weight increased significantly (p <0.05). However, the treatment of wheat seedlings exposed to drought stress with suitable doses of laser irradiation plus exogenous CAT (L + P + C), AsA (L + P + A) or DPI (L + P + Protective effect of seedlings. Thus, laser pretreatment played a protective role on the physiological and biochemical level of plants mainly through the induction of endogenous H2O2 production, and the formation of H2O2 was related to the NADPH oxidase pathway.