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采用对峙培养法筛选了三桠苦(Evodia lepta)内生真菌对香蕉炭疽病菌(Colletotrichum musae)、芒果炭疽病病菌(C.gloeosporioides)、橡胶炭疽病病菌(C.gloeosporioides)、香蕉枯萎病病菌(Fusarium oxysporum f.sp.cubense)4号生理小种、芒果蒂腐病病菌(Botryodiplodia theobromae)、椰子灰斑病病菌(Pestalotiapalmarum)的拮抗活性,并通过抑制菌圈法明确了发酵液中拮抗菌株活性产物存在位置和用生长速率法测定了其抑菌活性物质的毒力。结果表明,三桠苦内生真菌中有10株菌株具有拮抗活性,其中SCK-Y9拮抗效果最明显。该菌株抑菌活性物质分泌于胞外发酵液,发酵液上清液氯仿萃取物对供试6种靶标菌抑制中浓度均小于0.5 mg/mL。其中,对香蕉炭疽病病菌最敏感,EC50为0.010 0 mg/mL;芒果炭疽病病菌敏感性最差,EC50为0.413 1 mg/mL。
The antagonistic activity of endophytic fungi (Evodia lepta) to Colletotrichum musae, C. gloeosporioides, C. gloeosporioides, Fusarium oxysporum f.sp.cubense) 4, Botryodiplodia theobromae and Pestalotia palmarum, and the activity of antagonistic strains in the fermentation broth was clarified by inhibition of the bacterial loop The existence position of the product and the growth rate method were used to determine the virulence of the antibacterial active substance. The results showed that 10 strains of endophytic fungi have antagonistic activity, of which SCK-Y9 antagonistic effect is the most obvious. The antibacterial active substance of the strain was secreted in the extracellular fermentation broth. The medium concentration of the chloroform extract of the supernatant of the fermentation broth was less than 0.5 mg / mL for the six target bacteria tested. Among them, the most sensitive to banana anthracnose pathogen, EC50 0.010 0 mg / mL; mango anthracnose pathogenic bacteria the worst sensitivity EC50 of 0.413 1 mg / mL.