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为了从分子水平上研究陆地棉矮秆突变体Ari1327的矮化机理,本研究以矮秆突变体Ari1327、野生型Ari971和高秆突变体Ari3697的茎尖为材料,建立3个cDNA文库,用Illumina HiSeqTM2000系统对3个材料的茎尖cDNA进行转录组测序。3个文库测序共得4.9 G数据量,拼接得到Unigene 70877个。通过矮化突变体Ari1327与野生型Ari971和高秆突变体Ari3697两个文库的差异筛选,得到13919个与矮化相关的差异表达基因,其中5406个表现上调,8513个表现下调。GO功能和KEGG通路富集发现,差异基因在植物激素信号转导途径显著富集。通过实时荧光定量PCR(qRT-PCR)验证,推测Ari1327的矮化可能与赤霉素和生长素2种激素的信号转导及互作有关。转录组测序得到的大量差异基因,为深入研究棉花的矮化机理具有重要参考价值,同时为棉花的矮化育种工作奠定了基础。
In order to study the dwarfing mechanism of Ari1327, a dwarf mutant of Upland cotton at the molecular level, three cDNA libraries were constructed using stem tips of dwarf mutant Ari1327, wild type Ari971 and high-stalk mutant Ari3697, The HiSeqTM2000 system transcribes the shoot tip cDNAs from three materials. A total of 4.9 G data was obtained from sequencing of 3 libraries, and 70877 Unigene were spliced. 13,919 differentially expressed genes related to dwarfism were obtained by the differential screening of two libraries of the dwarf mutant Ari1327 and the wild type Ari971 and the high stalk mutant Ari3697, of which 5406 were up-regulated and 8513 were down-regulated. The GO function and KEGG pathway enrichment revealed that the differentially expressed genes were significantly enriched in plant hormone signaling pathways. Real-time quantitative PCR (qRT-PCR) showed that the dwarfing of Ari1327 might be related to the signal transduction and interaction of gibberellin and auxin. Transcriptome sequencing of a large number of different genes obtained for in-depth study of dwarfing mechanism of cotton has an important reference value, while cotton dwarf breeding laid the foundation.