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为研究低磷胁迫(LP)、正常条件(HP)2个处理对转Bt基因玉米和郑单958根系形态以及磷利用效率的影响。以转Bt基因玉米(Bt799)和受体郑单958为试验材料,进行温室水培试验。研究证明:1)低磷胁迫后Bt799的根长、根鲜重和根冠比升高幅度高于郑单958,就总根长、根系投影面积总和、根表面积、根平均直径、单位立方米总根长和根总容积而言,Bt799玉米的LP/HP是郑单958玉米LP/HP的1.13、1.19、1.33、1.18、1.14和1.55倍。2)低磷胁迫降低各叶位Bt蛋白量,根部除根基处1~7cm Bt蛋白量上升以外,其余根系均下降。在正常处理下,Bt799全株和根系Bt蛋白总量为1 750和423ng,低磷处理下Bt799分别为1 470和784ng。3)低磷胁迫下2种玉米叶片和根系磷含量和磷积累量显著降低,各叶位磷占总磷比例显著下降,但根系磷占总磷比例反而升高。研究表明,Bt799通过根长、根表面积和根系直径等形态变化来优化其空间分布构型,吸收更多的磷元素,但向叶片转运磷元素的能力较弱。
In order to study the effects of low phosphorus stress (LP) and normal conditions (HP) on root morphology and phosphorus use efficiency of transgenic Bt maize and Zhengdan 958. Bt transgenic maize (Bt799) and receptor Zhengdan 958 were used as experimental materials to conduct greenhouse hydroponic experiments. The results showed that: 1) The root length, root fresh weight and root / shoot ratio of Bt799 increased more slowly than that of Zhengdan 958 after low-P stress. The total root length, root projected area, root surface area, root mean diameter, Total root length and total root volume, LP / HP of Bt799 maize was 1.13, 1.19, 1.33, 1.18, 1.14 and 1.55 times that of Zhengdan 958 maize LP / HP. 2) Under low-phosphorus stress, the amount of Bt protein in leaves decreased, and the amount of Bt protein in roots increased by 1 ~ 7cm Bt. Under normal conditions, the total amount of Bt protein in whole plant and root of Bt799 was 1 750 and 423 ng, while Bt799 in low phosphorus treatment was 1 470 and 784 ng respectively. 3) Under low-P stress, the content of P and P in two kinds of maize leaves and roots decreased significantly, while the percentage of phosphorus in all leaves decreased significantly, but the proportion of total phosphorus in roots increased. The results showed that Bt799 optimized the spatial distribution of root morphology, root surface area and root diameter by absorbing more phosphorus, but its ability to transport phosphorus was weaker.