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目的了解集中空调通风系统送风中溶血性链球菌的污染和菌种的分布特征。方法于2008—2010年夏季期间4次对福建省属直管的9家大型宾馆集中空调采集的送风口空气样品进行溶血性链球菌检测,依据卫生部《公共场所集中空调通风系统卫生规范》的采样和检测方法,确定为溶血性链球菌的菌株,经纯培养后进一步采用VITEK全自动微生物鉴定仪鉴定。结果共分离到溶血性链球菌24株,β溶血性链球菌17株,包括4个种(无乳链球菌、停乳链球菌、马链球菌、酿脓链球菌);α溶血性链球菌7株,也包括4个种(肺炎链球菌、缓症链球菌、口腔链球菌、乳房链球菌)。菌株数从2008年8月首次监测时的15株降至2009年的5株、2010年的4株。结论福建省属直管的9家大型宾馆集中空调通风系统存在不同程度的溶血性链球菌污染,且经分离鉴定的菌株均是比较典型的致病代表株,经加强对公共场所集中空调的定期清洗和消毒等监管后,其污染程度有下降的趋势。
Objective To understand the pollution and distribution of hemolytic streptococcus in centralized air conditioning and ventilation system. Methods During the summer of 2008-2010, hemolytic streptococcus was detected in air samples collected from nine air-conditioned centralized air-conditioners in Fujian Province. According to the Ministry of Health’s “Code of Hygienic Practice for Central Air Conditioning and Ventilation System in Public Places” Sampling and testing methods, identified as hemolytic streptococcus strains, after pure culture further VITEK automatic microbial identification instrument identified. Results 24 strains of hemolytic streptococcus and 17 strains of β-hemolytic streptococcus were isolated, including 4 species (Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus equi, Streptococcus pyogenes), α-hemolytic streptococcus Strains also included 4 species (Streptococcus pneumoniae, Streptococcus mitis, Streptococcus oralis, Streptococcus uberis). The number of strains dropped from 15 at the first monitoring in August 2008 to 5 in 2009 and 4 in 2010. Conclusions There are different levels of hemolytic streptococcus contamination in the centralized air-conditioning and ventilation systems of nine large hotels in Fujian province which are under direct management. The strains isolated and identified are typical representatives of pathogenic strains. After being strengthened regularly on central air conditioners in public places, After cleaning and disinfection and other regulatory, the degree of pollution has a downward trend.