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目的:研究麻杏石甘汤对内毒素(LPS)致热模型大鼠血浆中内源性物质的影响。方法:25只大鼠随即分为空白组、致热模型组、低剂量组、中剂量组、高剂量组,每组5只。模型组及高、中、低剂量组动物分别以20μg.kg-1体重的剂量尾静脉注射10μg.mL-1内毒素溶液,空白组同法给予生理盐水(NS)。造模后6h内测量大鼠体温,6 h后,心脏取血。血浆预处理后,HPLC/MS/MS检测血浆中物质,PCA,OSC-PLS分析数据,分析潜在生物标记物。结果:各剂量组的最大体温上升高度ΔTmax与模型组比较差异均有显著性(P<0.05);在前两个主成分得分散点图中,中剂量、高剂量麻杏石甘汤对LPS致热大鼠血浆中内源性物质的影响较低剂量的影响要大,苹果酸,L-phenylalanine等物质在各组实验动物体内发生了相应的变化。结论:实验剂量麻杏石甘汤明显影响LPS致热大鼠血浆中内源性物质状态,苹果酸,L-phen-ylalanine可能是麻杏石甘汤对LPS致热大鼠血浆中内源性物质的影响的生物标记物。
Objective: To study the effects of Maxingshigan Decoction on the endogenous substances in the plasma of rats induced by endotoxin (LPS). Methods: 25 rats were randomly divided into blank group, induced model group, low dose group, middle dose group and high dose group, with 5 rats in each group. The model group and the high, middle and low dose groups were given 10μg.mL-1 endotoxin solution by tail vein injection at a dosage of 20μg.kg-1 respectively, while the blank group was given NS. Rats were measured body temperature within 6h after modeling, and blood was taken from the heart after 6 hours. After plasma preconditioning, plasma samples, PCA, OSC-PLS analysis data were analyzed by HPLC / MS / MS to analyze potential biomarkers. Results: The maximum body temperature rise height (ΔTmax) in each dose group was significantly different from that in model group (P <0.05). In the first two principal component score scatter plots, middle and high dose Maxing Shigan Decoction had no significant effect on LPS The effect of endogenous substances in the plasma of rats induced by fever was greater than that of lower dose. The substances such as malic acid and L-phenylalanine changed correspondingly in experimental animals. Conclusion: The experimental dose of Maxingshigan Decoction significantly affect the endogenous plasma LPS-induced substance state, malic acid, L-phen-ylalanine may be Maxing Shigan Decoction induced LPS-induced plasma endogenous Biomarkers of material effects.