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目的利用PCR-DGGE方法探讨戴用牙齿固定矫治器对口腔菌群的影响。方法从20例健康的牙齿正常个体和30例戴用固定矫治器的正畸患者的唾液中提取细菌基因组总DNA,利用聚合酶链式反应—变形梯度凝胶电泳(PCR-DGGE)方法获取口腔菌群的分子指纹图谱,对其进行相似性和多样性的分析以及优势条带的序列分析。结果 PCR-DGGE可明确将正常个体和正畸患者分成2个簇,且正畸患者口腔菌群的多样性明显增加,优势菌群发生转变,序列分析表明口腔链球菌数量明显减少,假单胞菌成为优势菌型。结论戴用固定矫治器能改变口腔菌群分布,引起口腔中有益菌的减少,促使致病菌增长,导致口腔菌群失调。
OBJECTIVE: To evaluate the effect of wearing orthodontic appliances on oral flora by PCR-DGGE. Methods Total genomic DNA of bacterial genomic DNA was extracted from saliva of 20 healthy normal individuals and 30 orthodontic patients with fixed appliances. The oral cavity was obtained by polymerase chain reaction-modified gradient gel electrophoresis (PCR-DGGE) The molecular fingerprinting of the microflora, its similarity and diversity analysis, as well as the analysis of the dominant band sequence. Results PCR-DGGE could clearly divide normal individuals and orthodontic patients into two clusters, and the diversity of oral flora in orthodontic patients was significantly increased and the dominant flora was changed. Sequence analysis showed that the number of Streptococcus oralis was significantly decreased, Bacteria become dominant bacteria. Conclusion wearing a fixed appliance can change the distribution of oral flora, causing the reduction of beneficial bacteria in the mouth, to promote the growth of pathogens, resulting in oral flora imbalance.