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本文应用组织培养、免疫细胞化学和电生理方法研究了体外培养条件下小脑髓鞘形成及电生理表现。结果表明，体外培养１３ｄ时，电镜下即可见到轴突周围有少量髓鞘板层。培养１４ｄ时，光镜下观察到，髓鞘为两条平行双折光结构，主要分布在髓质；在皮质和核区有髓鞘碱性蛋白阳性的少突胶质细胞集中分布。体外培养２４ｈ，在小脑皮质可记录到频率约为８０～１１０次／ｓ，幅值０．２ｍＶ的自发放电；至９ｄｉｖ时，可记录到较大的自发放电，频率约为４０～６０次／ｓ，幅值０．６ｍＶ。本文对体外培养条件下中枢神经系统髓鞘形成及小脑发育各阶段电生理表现进行了讨论。
In this study, we used tissue culture, immunocytochemistry and electrophysiological methods to study the cerebellarmyelination and electrophysiological findings in vitro. The results showed that a small amount of myelin sheath around the axon could be seen under electron microscope 13 days after cultured in vitro. At 14 days of culture, myelin was observed as two parallel birefringent structures under light microscopy, mainly distributed in the medulla; in the cortex and nucleus, myelin basic protein-positive oligodendrocytes were concentratedly distributed. In vitro cultured in the cerebellar cortex can be recorded to the frequency of about 80 ~ 110 times / s, amplitude 0.2mV spontaneous discharge; to 9div, can be recorded to a larger spontaneous discharge, the frequency of about 40 to 60 times / s, amplitude 0.6mV. In this paper, the myelination of the central nervous system and the electrophysiological performance of cerebellar development in vitro were discussed.