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目的 研究巯基乙醇和维生素C能否阻断镉对红细胞SOD的抑制。方法 用羟胺发色法分别对对照组红细胞 ,CdCl2 孵育红细胞 ,巯基乙醇与CdCl2 孵育红细胞 ,维生素C与CdCl2 孵育红细胞的溶血液SOD活性进行了测定。结果 ①镉孵育红细胞SOD活性显著低于对照组 (P <0 0 1) ;②巯基乙醇与小剂量镉 (0 11μmol/Lblood)共同孵育红细胞SOD活性升高 (P <0 0 5 ) ;③维生素C与镉孵育红细胞SOD活性显著高于镉孵育红细胞组 (P <0 0 1)。结论 ①加入到血中的镉易进入红细胞中 ,抑制红细胞SOD ,且红细胞中的镉浓度随血镉浓度的增加而增加 ;②巯基乙醇能改善低浓度血镉对红细胞SOD的抑制 ;③维生素C可明显阻断镉对红细胞SOD的抑制
Objective To investigate whether mercaptoethanol and vitamin C can block the inhibitory effect of cadmium on erythrocyte SOD. Methods Hydroxylamine colorimetry was used to measure the activity of erythrocytes in control group, erythrocytes incubated with CdCl2, erythrocytes incubated with mercaptoethanol and CdCl2, and SOD activity in hemolysate of erythrocytes incubated with vitamin C and CdCl2. Results ① The activity of SOD in cadmium-incubated erythrocytes was significantly lower than that in the control group (P <0.01); ② The activity of SOD was increased (P <0.05) in the co-incubated erythrocytes with mercaptoethanol and low dose cadmium (0 11μmol / Lblood) C and cadmium incubated erythrocyte SOD activity was significantly higher than that of cadmium-incubated erythrocyte group (P <0.01). Conclusions ① Cadmium added to the blood easily enters the red blood cells and inhibits the SOD of the red blood cells, and the cadmium concentration in the red blood cells increases with the increase of the blood cadmium concentration; ② Mercaptoethanol can improve the inhibitory effect of low blood cadmium on the red blood cell SOD; ③ Vitamin C Can significantly block the inhibition of cadmium on erythrocyte SOD