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目的研究气态苯(106mg/m3)吸入染毒大鼠致组织器官氧化损伤作用,探讨苯对机体的脂质过氧化作用及机体的抗氧化损伤机制。方法通过建立气态苯动式吸入染毒大鼠的实验动物模型,连续染毒7d,每天4h;染毒结束后,采用分光光度法测定各组织器官(肺、脑、肝和外周血)中还原型谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GSHPX)活力、超氧化物歧化酶(SOD)活性及脂质过氧化产物丙二醛(MDA)含量。结果苯吸入组大鼠肺、肝、脑组织及外周血均表现不同程度的氧化损伤,其中大鼠肺组织SOD活力,肝组织GSHPX和MDA及脑组织的MDA含量与对照组比较差异均有统计学意义(P<0.05),外周血GSH、GsHPx和MDA及TS0D水平与对照组比较也表现增加趋势。结论推测苯可诱发大鼠机体发生一系列的氧化应激损伤,并且具有多器官性的特点。
Objective To study the oxidative damage of tissues and organs induced by inhaled gaseous benzene (106mg / m3), and to explore the lipid peroxidation of benzene and the mechanism of antioxidative damage. Methods The experimental animal model of gaseous benzene inhalation was established by continuous inhalation of benzene for 7 days and daily for 4 hours. After the end of the exposure, spectrophotometry was used to determine the contents of various components in tissues and organs (lung, brain, liver and peripheral blood) (GSH), glutathione peroxidase (GSHPX) activity, superoxide dismutase (SOD) activity and lipid peroxidation product malondialdehyde (MDA) content. Results The lungs, liver, brain and peripheral blood of benzene inhalation rats showed different degrees of oxidative damage. The SOD activity in lung tissue, GSHPX and MDA in liver tissue and the content of MDA in brain tissue of rats in benzene inhalation group were statistically different from those in control group (P <0.05). The levels of GSH, GsHPx, MDA and TS0D in peripheral blood also showed an increasing trend compared with the control group. Conclusion It is speculated that benzene can induce a series of oxidative stress injury in rats and has the characteristics of multiple organs.