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目的观察多发性脑梗塞大鼠海马CA3区神经元的SP和SPR变化及神经生长因子(nervegrowthfactor,NGF)、精制蝮蛇抗栓酶(Svate-3)联合应用对MCI(multi-cerebralInfarction,MCI)的影响,为进一步探讨MCI发病时的细胞内机制和临床治疗提供了实验依据。方法选用Wistar大鼠30只,随机分为正常对照组、实验对照组和实验组。应用微栓子栓塞阻断法建立MCI缺血性动物模型,借用组织化学的方法结合显微图像分析,观察大鼠海马CA3区神经元的SP和SPR改变,并且对比观察NGF,Svate-3对MCI海马CA3区神经元的作用。结果SP及SPR阳性反应物平均灰度值组间比较,实验对照组比正常对照组升高分别为(202±10),(127±7)和(168±8),(95±9),P<0.01。结论SP及SPR可能参与MCI病理生理过程,NGF和Svate-3在缺血状态下可以减轻神经元的损伤,并能提高其存活能力。
Objective To observe the effects of nerve growth factor (NGF) and Svate-3 on the changes of SP and SPR in hippocampal CA3 subregion of multiple cerebral infarction rats and the effect of MCI (multi-cerebral infraction) In order to further explore the pathogenesis of MCI intracellular mechanism and clinical treatment provided an experimental basis. Methods Thirty Wistar rats were randomly divided into normal control group, experimental control group and experimental group. The MCI-ischemic animal model was established by micro-embolism occlusion method. The histochemical and microscopic images were used to observe the changes of SP and SPR in hippocampal CA3 neurons of rats and the comparison of NGF and Svate-3 Effect of neurons in hippocampal CA3 area of MCI. Results Compared with the normal control group, the mean gray values of SP and SPR positive reactors were (202 ± 10), (127 ± 7) and (168 ± 8), (95 ± 9) and P <0.01. Conclusion SP and SPR may participate in the pathophysiological process of MCI. NGF and Svate-3 can reduce neuronal damage and increase their viability in ischemic state.