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为了解连续施药对番茄灰葡萄孢对咯菌腈敏感性的影响,在温室条件下,从灰霉病发生初期开始,按推荐剂量(有效成分67.5 g/hm2)定期向番茄苗喷施咯菌腈,通过菌丝生长速率法检测灰葡萄孢对咯菌腈的敏感性。结果表明,在番茄1个生长季节中连续施药7次后,病菌对咯菌腈仍表现敏感,其EC50值在0.004 7～0.046 2μg/mL之间。在含咯菌腈的PDA培养基上对30个灰葡萄孢敏感菌株进行药剂驯化7代后,共产生2个抗药性突变体(Rg-12和Rdz-28),其中Rg-12对咯菌腈的EC50值是其亲本菌株g-12的46倍,Rdz-28的EC50值大于500μg/mL,2个抗性突变体在菌丝生长速率、产孢量和产菌核能力方面均显著低于其亲本菌株。推测灰葡萄孢可能不易对咯菌腈产生抗性,咯菌腈可作为防治番茄灰霉病的理想候选药剂。
In order to understand the effect of continuous spraying on susceptibility to fludioxonil in Botrytis cinerea, the tomato seedlings were sprayed at the recommended dose (active ingredient 67.5 g / hm2) Bacterial nitrile, the sensitivity of Botrytis cinerea to fludioxonil was determined by mycelial growth rate method. The results showed that the pathogen was susceptible to fludioxonil after seven consecutive application of tomato in one growth season with EC50 between 0.004 7 and 0.046 2 μg / mL. After resistant to 30 strains of Botrytis cinerea on PDA media containing flumicitrobenzene for 7 generations, two drug-resistant mutants (Rg-12 and Rdz-28) were produced, of which Rg-12 The EC50 value of nitrile was 46 times that of its parental strain g-12, and the EC50 value of Rdz-28 was greater than 500μg / mL. The two resistant mutants were significantly lower in mycelial growth rate, sporulation and nuclear producing capacity In its parent strain. It is speculated that Botrytis cinerea may not be resistant to fludioxonil, and fludioxonil may be an ideal candidate for control of Botrytis cinerea.