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为确诊某猪群猪痢疾短螺旋体病,本实验对疑似病例剖检采集结肠粘膜样品,涂布于TSA血平板,37℃严格厌氧培养5 d,进行细菌分离鉴定,获得β溶血的分离株。分离株通过瑞氏-吉姆萨染色、扫描电镜观察和PCR检测,鉴定为猪痢疾短螺旋体。病理组织学显示:结肠呈现严重的卡他性炎症、产生大量粘液、血管充出血和粘膜坏死。高倍镜下显示:肠上皮细胞之间粘结疏松,胶原纤维溶解、断裂。结果表明,该猪群感染猪痢疾短螺旋体,病原菌侵害结肠和盲肠粘膜组织,造成严重损伤。
In order to diagnose a swine swine herpes simplex B. tuberculosis, in this experiment, colonic mucosa samples were collected from the suspected cases and applied to TSA blood plate. After strict anaerobic culture at 37 ° C for 5 days, the bacteria were isolated and identified to obtain the β-hemolysis isolates . The isolate was identified as B. hyodysenteriae by Wright-Giemsa staining, scanning electron microscopy and PCR. Histopathology showed that: the colon presents a serious catarrhal inflammation, resulting in a large number of mucus, blood vessels bleeding and mucosal necrosis. High magnification showed: loose bonding between intestinal epithelial cells, collagen fibers dissolved, broken. The results showed that the swine herd was infected with B. hyodysenteriae and the pathogens invaded the colon and cecum mucosa, resulting in serious damage.