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目的:评价白花蛇舌草多糖的体外抗氧化能力。方法:以热水回流提取法(料水比1∶31.26,84.71℃下提取2.07h,提取2次)从白花蛇舌草(HDW)提取的多糖为材料,通过1,1-二苯基-2苦肼基自由基(DPPH)清除率、总的抗氧化活性和还原能力测定等体外抗氧化试验来评价白花蛇舌草多糖的体外抗氧化能力。结果:白花蛇舌草多糖清除DPPH自由基、总的抗氧化活性和还原能力均随多糖浓度的增加而上升;1mg/mL的多糖对DPPH自由基的清除率高达82.66%;1mg/mL多糖的总的抗氧化活性在695nm下吸光值为1.641;1mg/mL多糖的还原能力在700nm下吸光值为0.773。结论:白花蛇舌草多糖有较强的抗氧化能力,对体外自由基有较好的清除作用。
Objective: To evaluate the antioxidant capacity of Hedyotis diffusa polysaccharides in vitro. Methods: The polysaccharides extracted from Hedyotis diffusa Willd. (HDW) were extracted by hot water extraction (ratio of material to water 1:31.26, extraction 2.07h at 44.71 ℃ for 2 times) 2 scavenging free radical (DPPH) scavenging rate, total antioxidative activity and reducing ability were tested in vitro to evaluate the antioxidant activity of Hedyotis diffusa Willd polysaccharides in vitro. Results: The DPPH radical scavenging activity of Hedyotis diffusa Willd polysaccharides was abolished, and the total antioxidative activity and reducing ability increased with the increase of polysaccharide concentration. The scavenging rate of DPPH radical at 1 mg / mL was up to 82.66% The total antioxidant activity at 695nm absorbance was 1.641; 1mg / mL polysaccharide reduction at 700nm absorbance of 0.773. Conclusion: Hedyotis diffusa polysaccharides has a strong antioxidant capacity, free radical scavenging effect in vitro.