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为了探讨在发育过程中鸡胚脊髓背角提取液的神经营养活性作用 ,分别取 Hamburger3 0期和 40期鸡胚的脊髓背角组织制成条件培养液 ,以 Hanks平衡盐溶液代替背角提取液设置对照组。实验组分别培养了两时相的脊神经节及其神经元。培养48h时测量各组每个脊神经节神经元的神经突起平均长度及神经元存活数。结果 :两时相实验组的神经元存活数量均明显大于对照组 (P<0 .0 5 ) ;而神经突起长度仅 40期组者大于对照组 (P<0 .0 5 )。另外 ,40期组脊神经节神经元的神经突起长度及神经元存活数量也明显高于 3 0期者。提示 ,两时相脊髓背角内均存在某些初级感觉神经元诱向因子。但在不同的发育阶段脊髓背角的神经营养活性却有变化。
In order to explore the role of neurotrophic activity in the chicken embryo spinal cord dorsal horn extract during development, the spinal cord dorsal horn tissue of Hamburger 3 0 and 40 embryos were made into conditioned medium, Hanks balanced salt solution instead of dorsal horn extract Set the control group. Experimental group were cultured in two phases of spinal ganglia and neurons. The mean length of neurites and the number of neurons surviving in each spinal ganglion neuron of each group were measured at 48 hours of culture. Results: The number of neurons survived in the two experimental groups was significantly larger than that in the control group (P <0.05), while the number of neurites in the experimental group was only 40 (P <0.05). In addition, neurite length and number of neurons surviving in group 40 spinal ganglion neurons were significantly higher than those in stage 30. Tip, two-phase spinal cord dorsal horn exists in some primary sensory neurons induced factor. However, there are changes in the neurotrophic activity of spinal dorsal horn at different developmental stages.