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为探讨再障发病机制中各种造血生长因子（ＨＧＦｓ）对其造血干细胞的影响，了解各种ＨＧＦｓ对造血干细胞生长、增殖的具体作用及相互间的协同作用，采用甲基纤维素半固体体外短期培养及长期液体培养的方法，观察ＨＧＦｓ如粒一巨噬细胞集落刺激因子（ＧＭ—ＣＳＦ）、粒细胞集落刺激因子（Ｇ—ＣＳＦ）、白细胞介素－３（ＩＬ—３）、ＩＬ—６、干细胞因子（ＳＣＦ）、促红细胞生成素（Ｅｐｏ）以及ＨＧＦｓ不同组合方式对再障患者骨髓造血干细胞增殖、分化的调控作用。发现再障患者的克隆形成细胞（ＣＦＣ）形成巨噬细胞粒细胞集落形成单位（ＣＦＵ—ＧＭ）和红系爆发式集落形成单位（ＢＦＵ—Ｅ）的能力受限，但大部分患者（１０／１２）骨髓中长期培养起始细胞（ＬＴＣ—ＩＣ）接近正常，提示再障的造血干细胞可能在分化成熟过程中受到阻碍所致。此外，各种ＨＧＦｓ均有不同程度的提高造血干细胞形成集落的能力，且不同ＨＧＦｓ之间对再障患者ＣＦＣ形成ＣＦＵ—ＧＭ、ＢＦＵ—Ｅ的能力同样具有协同作用，其中以ＳＣＦ、ＩＬ—３和ＣＭ—ＣＳＦ的联合应用更为显著（Ｐ＜０．００１）。
To investigate the effect of various hematopoietic growth factors (HGFs) on hematopoietic stem cells in the pathogenesis of aplastic anemia, and to understand the specific effects and mutual synergistic effects of various HGFs on the growth and proliferation of hematopoietic stem cells, (G-CSF), interleukin-3 (IL-3) and interleukin-3 (IL-3) 6, stem cell factor (SCF), erythropoietin (Epo) and HGFs different combinations of aplastic anemia patients with bone marrow hematopoietic stem cell proliferation and differentiation regulation. The ability of clonogenic cells (CFCs) to form macrophage granulocyte colony forming units (CFU-GMs) and erythroid blastfold colony forming units (BFU-E) was found to be limited in patients with aplastic anemia, but most patients (10 / 12) The bone marrow long-term culture of starting cells (LTC-IC) close to normal, suggesting that aplastic anemia hematopoietic stem cells may be obstructed in the process of differentiation and development. In addition, all kinds of HGFs increased the ability of forming hematopoietic stem cells to form colonies in varying degrees, and the synergistic effects of different HGFs on the ability of CFC to form CFU-GM and BFU-E also had synergistic effects. Among them, SCF, IL-3 Combined with CM-CSF was more significant (P <0.001).