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以十和田/昆明小白谷225个F14家系为作图群体,在云南省弥勒县(正常生长环境)、嵩明县(自然低温胁迫环境)、丽江市(自然低温胁迫环境)等3个试点不同年份共5种不同生长环境下进行了水稻主穗和分蘖穗穗伸出度的异地鉴定,并利用SSR标记对水稻穗伸出度进行了QTL分析。检测结果表明,在5种不同的生长环境下共检测到12个与水稻穗伸出度相关的QTL,分别分布于第1(2个QTLs)、2、4、6(3个QTLs)、7(3个QTLs)、9(2个QTLs)号染色体,对表型的贡献率为3.72%~22.17%。其中与主穗穗伸出度相关的QTL共11个,与分蘖穗穗伸出度相关的QTL共7个,其中6个在主穗和分蘖穗上均检测到。在与主穗穗伸出度相关的11个QTL中,q PE-7-1在4种环境下均被检测到,解释的表型变异为9.49%~22.17%;q PE-1-1、q PE-1-2、q PE-6-1和q PE-9-2 4个QTL在2种环境下均被检测到。在与分蘖穗穗伸出度相关的7个QTL中,q PE-1-2、q PE-7-1和q PE-6-1 3个QTL在2种环境中均被检测到,解释的表型变异率分别为4.35%~12.64%、13.22%~20.89%和11.49%~15.73%。
A total of 225 F14 pedigrees from Shidubaowan and Kunming were used as the mapping population. Three pilot plots were conducted in three pilot sites, namely, Mile County (normal growth environment), Songming County (natural low temperature stress environment) and Lijiang (natural low temperature stress environment) in Yunnan Province. A total of 5 different growth environments were conducted to identify the spikelet heading and panicle elongation in rice. SSR markers were used to analyze the QTLs for panicle elongation in rice. The results showed that 12 QTLs related to panicle elongation were detected in 5 different growth environments, which were found in QTLs 1, 2, 4 and 6 (3 QTLs), 7 (3 QTLs) and 9 (2 QTLs) chromosomes, which contributed 3.72% ~ 22.17% to the phenotype. Among them, there were 11 QTLs related to main spike extension and 7 QTLs related to tillering spike extension, of which 6 were detected on both main and tillering spikes. Of the 11 QTLs associated with main spike spike, qPE-7-1 was detected in all four environments, accounting for 9.49% -22.17% of phenotypic variation; q PE-1-1, Four QTLs of q PE-1-2, q PE-6-1 and q PE-9-2 were detected in both environments. Among the seven QTLs related to panicle spike elongation, three QTLs of q PE-1-2, q PE-7-1 and q PE-6-1 were detected in both environments, The phenotypic variation rates were 4.35% ~ 12.64%, 13.22% ~ 20.89% and 11.49% ~ 15.73%, respectively.