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1 材料 动物:DHP豚鼠,雄性,体重250±27g,上海西普尔—必凯实验动物有限公司提供,合格证号:0001750。饲料:豚鼠颗粒饲料加青菜。蛙鸣粉:经粉碎过100目筛,制成水煎剂,小鼠1g/ml,豚鼠3.3/ml。卵蛋白:中科院上海生化所东风生化技术公司提供,批号9503041。2 方法2.1 模型的建立 取雄性豚鼠按体重随机分3组,正常对照组、模型组、蛙鸣粉组,哮喘模型复制参照国际上常用的方法。用1％卵蛋白生理盐水1ml腹腔注射,免疫后第7d开始连续灌胃给药,每d1次,每次2ml。免疫2w后将致敏豚鼠放入玻璃罩内,雾化吸入1％卵蛋白生理盐水15s,井在雾室中放置至
1 Materials Animals: DHP guinea pigs, male, body weight 250±27g, provided by Shanghai Sea Pool-Bikai Laboratory Animal Co., Ltd., certificate No.: 0001750. Feed: Guinea pig pellet feed plus vegetables. Frogming powder: After smashing 100 mesh sieve, make water decoction, mouse 1g/ml, guinea pig 3.3/ml. Egg protein: provided by Dongfeng Biochemical Technology Co., Ltd., Shanghai Institute of Biochemistry, Chinese Academy of Sciences, batch number 9503041. 2 Method 2.1 Establishment of model Male guinea pigs were randomly divided into 3 groups according to body weight: normal control group, model group, and frogming powder group. The asthma model was replicated internationally. Commonly used methods. Intraperitoneal injection of 1% ovalbumin saline 1ml, 7 days after immunization, continuous intragastric administration, every d1, each 2ml. After 2 weeks of immunization, the sensitized guinea pigs were placed in a glass cover and inhaled with 1% egg albumin saline for 15 seconds. The wells were placed in the fog chamber until