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目的 :探讨 Ki- 6 7抗原和微血管密度 (MVD)在膀胱移行细胞癌中的表达及临床意义 ,并评价两者的相互关系。方法 :利用鼠抗人 Ki- 6 7抗原单克隆抗体和鼠抗人 F 因子相关抗原 (F RAg)单克隆抗体对 6 5例膀胱移行细胞癌和 8例正常膀胱黏膜进行免疫组化染色。结果 :Ki- 6 7与 MVD在癌组织中表达均显著高于正常黏膜 (P<0 .0 1)。肿瘤中 Ki- 6 7指数和 MVD之间存在正相关性 ,两者的表达均与膀胱移行细胞癌的病理分级显著相关 , 级与 级、 级与 级之间存在显著差异 (P<0 .0 1) ;浸润性肿瘤组高于表浅肿瘤组 (P<0 .0 1) ;术后 2年随访复发组高于未复发组 (P<0 .0 1)。MVD与肿瘤大小有关 ,肿瘤直径大于 2 cm组 MVD高于肿瘤直径小于 2 cm组 (P<0 .0 5 )。结论 :Ki- 6 7的表达为膀胱移行细胞癌的恶性表型 ,且与膀胱肿瘤血管形成有关。Ki- 6 7指数和 MVD对评价膀胱移行细胞癌的生物学形为和预后判断具有重要意义
Objective: To investigate the expression of Ki-67 antigen and microvessel density (MVD) in transitional cell carcinoma of the bladder and its clinical significance, and to evaluate the relationship between the two. Methods: Totally 65 cases of bladder transitional cell carcinoma and 8 cases of normal bladder mucosa were stained immunohistochemically with monoclonal anti-human K-6 7 monoclonal antibody and mouse anti-human F factor-related antigen (F RAg) monoclonal antibody. Results: The expression of Ki- 6 7 and MVD in cancer tissues were significantly higher than that in normal mucosa (P <0.01). There was a positive correlation between Ki- 6 7 index and MVD in tumor, both of which were significantly correlated with the pathological grade of bladder transitional cell carcinoma (P <0. 0 1). The invasive tumor group was higher than the superficial tumor group (P <0.01). The follow-up of 2-year follow-up was higher in the recurrence group than in the non-recurrence group (P <0.01). The MVD was related to tumor size. The MVD of tumor diameter greater than 2 cm was higher than that of tumor diameter less than 2 cm (P <0.05). Conclusion: The expression of Ki-67 is a malignant phenotype of transitional cell carcinoma of bladder and is related to the angiogenesis of bladder tumor. Ki- 6 7 Index and MVD are Important for Evaluating the Biological Shape and Prognosis of Bladder Transitional Cell Carcinoma