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大鼠预饲高脂饲料（占日摄入热量的２０％、３５％和４５％）１月后，进行急性（－１５℃，２ｈ和慢性（０℃，３ｗｋ）冷暴露。测定心肌线粒体细胞色素Ｃ氧化酶（ＣＣＯ）及肝线粒体脂蛋白脂酶（ＬＰＬ）活性及耐寒力。结果如下：（１）急性冷暴露组ＣＣＯ及ＬＰＬ活性明显高于慢性冷暴露组及室温对照组（Ｐ＜０．０１）。慢性冷暴露组的ＬＰＬ活性亦高于室温对照组。（２）０℃、８ｈ冷暴露时直肠温度下降幅度明显小于食用普通低脂饲料的大鼠（Ｐ＜０．０１）。（３）２０％和３５％脂肪组在０℃、３ｗｋ慢性冷暴露过程中，体重的恢复早于４５％脂肪组。结果提示预饲１个月高脂饲料，有利于提高耐寒力和加速冷适应，脂肪产热比以２０％～３０％为宜。
Rats were pre-fed with high-fat diet (20%, 35% and 45% of daily calories) for 1 month and then subjected to acute (-15 ℃, 2h and chronic (0 ℃, 3wk) (CCO), lipoprotein lipase (LPL) activity and cold resistance of the liver.The results were as follows: (1) The activities of CCO and LPL in acute cold exposure group were significantly higher than those in chronic cold exposure group and room temperature control group (P < 0.01) .The LPL activity of chronic cold exposure group was also higher than that of room temperature control group. (2) The rectal temperature decreased significantly at 0 ℃ and 8h cold exposure (P <0.01) . (3) The weight recovery of 20% and 35% fat groups was earlier than that of 45% fat groups at 0 ℃ and 3 weeks of chronic cold exposure.The results suggested that feeding 1-month high-fat feed could improve cold resistance and accelerate Cold adaptation, fat to heat than 20% to 30% is appropriate.