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本文利用羧甲基羟胺（ＣＭＯ），在睾酮３位引入羧基，经Ｎ－羟基琥珀酰亚胺活化羧基后，与异鲁米诺衍生物（ＡＢＥＩ）脱水缩合，合成了睾酮的化学发光标记物（Ｔ─３－ＣＭＯ─ＡＢＥＩ）．并对该标记物进行了以下鉴定：（ｌ）ｍｐ．１２８～１３０℃；（２）硅胶ＧＦ－２５４薄层层析：展开剂为：氯仿：甲醇：氨水＝７５：３０：５，仅有一个斑点Ｒｆ＝０．８６；（３）紫外光谱：λＭｅＨＯｍａｘ：２０２，２３６，２８４，３１０ｎｍ；（４）快原子轰击质谱：Ｍ＋ｌ＝６２０该标记物的无水甲醇贮备液，在４℃下可长期稳定，工作试剂７２ｈ内未见发光效率下降，发光检测限达０．２１ｐｇ／管．
In this paper, carboxymethylhydroxylamine (CMO) was introduced into the carboxyl group of testosterone at position 3, and the carboxyl group was activated by N-hydroxysuccinimide. After dehydration and condensation with isoprenaminyl derivative (ABEI), the chemiluminescent label of testosterone (T ─ 3-CMO ─ ABEI). This marker was identified as: (l) mp. 128 ~ 130 ℃; (2) silica gel GF-254 TLC: developing solvent: chloroform: methanol: ammonia = 75: 30: 5, only one spot Rf = 0.86; (3) UV: λMeHOmax : 202,236,284,310 nm; (4) Fast atom bombardment mass spectrometry: M + l = 620 This indicator of anhydrous methanol stock solution, long-term stability at 4 ℃, working reagent 72h no luminous efficiency decline, luminescence detection Limit 0.21pg / tube.