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目的初探ARHI和突变型P53在人脑星形胶质细胞瘤中的表达及与恶性程度的相关性。方法采用免疫组织化学S-P法检测10例正常脑组织、30例低级别胶质瘤(Ⅰ、Ⅱ级)和39例高级别胶质瘤(Ⅲ、Ⅳ级)ARHI和突变型P53的表达情况,并分析二者之间的关系。结果与正常脑组织相比,ARHI在人脑胶质瘤中的表达显著降低,突变型P53在人脑胶质瘤中的表达显著增加。通过阳性区平均积分光密度定量分析,结果显示,正常组、低级别胶质瘤组和高级别胶质瘤组ARHI和突变型P53分别为(176.41±5.37)和(104.52±4.57)、(148.73±3.82)和(126.68±2.46)、(123.59±3.74)和(147.23±4.97),各组之间ARHI和突变型P53的测定值有统计学差异(P<0.01),并且二者在人脑胶质瘤中的表达呈负相关(r=-0.532,P<0.05)。结论 ARHI和突变型P53可能成为判断星形胶质细胞瘤恶性程度、侵袭性及预后的有效指标。
Objective To investigate the expression of ARHI and mutant P53 in human brain astrocytoma and its correlation with malignancy. Methods Immunohistochemical SP method was used to detect the expression of ARHI and mutant P53 in 10 cases of normal brain tissue, 30 cases of low grade gliomas (grade Ⅰ and Ⅱ) and 39 cases of high grade gliomas (Ⅲ and Ⅳ) And analyze the relationship between the two. Results Compared with normal brain tissue, the expression of ARHI in human glioma was significantly decreased, and the expression of mutant P53 in human glioma was significantly increased. Quantitative analysis of average integral optical density in the positive region showed that ARHI and mutant P53 in normal group, low grade glioma group and high grade glioma group were (176.41 ± 5.37) and (104.52 ± 4.57), (148.73 ± 3.82) and (126.68 ± 2.46), (123.59 ± 3.74) and (147.23 ± 4.97), respectively. There was a significant difference between ARHI and mutant P53 in each group (P <0.01) The expression of glioma was negatively correlated (r = -0.532, P <0.05). Conclusions ARHI and mutant P53 may be useful indicators of malignancy, invasiveness and prognosis of astrocytomas.