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目的 :观察姜黄素和阿霉素联合应用对体外培养的人肝癌细胞 SMMC- 772 1生长的影响。方法 :采用 MTT法应用 Webb系数判定 2药合用的效果 ,应用流式细胞仪和透射电镜观察药物对细胞周期分布及超微结构的影响。结果 :2种药物单独应用时随着药物剂量增加 ,其效应也增加 ;2药大剂量合用时可产生拮抗作用 ,小剂量合用时可产生协同作用。结论 :在体外 ,2 .5 mg/L～ 5 m g/L的姜黄素和 0 .12 5 mg/L～ 0 .5 mg/L的阿霉素联合应用 ,对 SMMC- 772 1具有协同抑制作用。姜黄素对阿霉素有化疗增效减毒作用
Objective : To observe the effect of curcumin combined with adriamycin on the growth of human hepatoma cells SMMC- 772 1 in vitro. Methods: The effect of two drugs was determined by using Webb coefficient by MTT method. Flow cytometry and transmission electron microscopy were used to observe the effects of drugs on cell cycle distribution and ultrastructure. RESULTS: The effects of two drugs with the increase of drug dose were increased when they were used alone; 2 antagonistic effects were produced when the drugs were combined in large doses, and synergistic effects were produced when the drugs were combined in small doses. Conclusion: In vitro, the combined application of curcumin of 2.5 mg/L to 5 mg/L and adriamycin of 0.125 mg/L to 0.5 mg/L has a synergistic inhibitory effect on SMMC-772 1. . Curcumin has a synergistic and attenuating effect on adriamycin