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目的制备聚乳酸一羟基乙酸共聚物(polylactide-co-glycolide,PLGA)包裹meta-四-(间羟基苯基)二氢卟吩[meta(tetrahydroxyphenyl)chlorin,m-THPC]的纳米型光敏剂,在体外研究其对人肝癌细胞光动力治疗(photodynamic therapy,PDT)作用。方法采用o/w的方法制备PLGA-m-THPC纳米微球,扫描电子显微镜下检测其粒径大小。荧光光谱仪检测其在含10%胎牛血清的细胞培养液中的吸收光谱。在人肝癌细胞株HepG2细胞的培养液内,分别加入不同浓度的PLGA-m-THPC,加红光照射后,观察细胞形态变化,并用细胞计数的方法测定细胞的存活率。结果PLGA-m-THPC纳米颗粒平均直径为180nm。PLGA-m-THPC在含血清培养液中的吸收光谱图显示其在652nm处有一个较强的吸收峰,并随着时间的推移吸光度增强。PLGA-m-THPC进入细胞主要聚集在胞浆,其对HepG2细胞的光敏杀伤作用随浓度的增加而增强。结论PLGA-m-THPC具有缓释、生物相容性好及光毒性低等优点,是一种理想的光敏剂。
OBJECTIVE To prepare nano-photosensitizers containing meta-tetrahydroxyphenyl chlorin (m-THPC) as polylactide-co-glycolide (PLGA) Its effect on photodynamic therapy (PDT) of human hepatoma cells was studied in vitro. Methods The PLGA-m-THPC nanospheres were prepared by o / w method. The size of the nanoparticles was detected by scanning electron microscopy. Fluorescence spectroscopy was used to detect the absorbance spectra of the cells in 10% fetal calf serum. Different concentrations of PLGA-m-THPC were added to the culture of HepG2 cells, and the changes of cell morphology were observed after adding red light. The cell viability was measured by cell counting method. Results The average diameter of PLGA-m-THPC nanoparticles was 180 nm. Absorption spectra of PLGA-m-THPC in serum-containing medium showed a strong absorption peak at 652 nm and increased absorbance over time. PLGA-m-THPC into the cells mainly in the cytoplasm, the phototoxicity of HepG2 cells increased with increasing concentration. Conclusion PLGA-m-THPC is an ideal photosensitizer with the advantages of sustained release, good biocompatibility and low phototoxicity.