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运动性疲劳与细胞凋亡时机体内环境的改变有雷同之处 ,通过对疲劳与细胞凋亡关系的研究 ,为运动性疲劳的发生机制提供理论依据。采用流式细胞仪 (FCM)检测肝、肌细胞Ca2 + 浓度及DNA倍体分析 ,末端转移酶标记 (TUNEL)技术检测细胞凋亡。结果表明 ,运动性疲劳后肌、肝细胞中Ca2 + 浓度显著增高P <0 .0 1;FCM检测肌、肝细胞实验组均出现凋亡峰 ;TUNEL染色显示 ,疲劳时肌、肝组织呈棕黄色的凋亡细胞比例明显增多 ;各组织SOD活性 12d组显著下降P <0 .0 1,MDA数量 12d组非常显著升高P <0 .0 1;研究认为 :细胞内Ca2 + 浓度显著增多、SOD与MDA比值变化是决定细胞凋亡或坏死的主要原因 ,细胞凋亡与运动性疲劳同步发生 ,诱导细胞发生凋亡的因素均会导致疲劳。
Exercise fatigue and apoptosis in vivo changes in the environment are similar, through the study of the relationship between fatigue and apoptosis, provide the theoretical basis for the mechanism of exercise-induced fatigue. Flow cytometry (FCM) was used to detect the concentration of Ca2 + in liver and muscle cells, DNA ploidy analysis and TUNEL assay. The results showed that the Ca2 + concentration in muscle and hepatocytes increased significantly after exercise fatigue (P <0.01), while apoptosis peak appeared in muscle and hepatocyte groups by FCM. The TUNEL staining showed that the muscle and liver tissues were brown Yellow apoptotic cells increased significantly; SOD activity in each group significantly decreased 12d group P <0. 01, 12d group of MDA was significantly increased P <0. 01; the study shows that: a significant increase in intracellular Ca2 + concentration, Changes in the ratio of SOD to MDA are the main determinants of apoptosis or necrosis. Apoptosis occurs concurrently with exercise-induced fatigue, and the factors inducing cell apoptosis may lead to fatigue.