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目的：评价聚合酶链反应（ＰＣＲ）法检测血清ＨＢＶＤＮＡ的临床意义及其优越性。方法：用ＰＣＲ法测定８１例血清ＨＢＶ标志物阳性的ＨＢＶＤＮＡ，并与固相放射免疫测定法作比较。结果：血清ＨＢＶＤＮＡ阳性率依次为：“大三阳”和ＨＢｃＡｇ（＋）组＞“大三阳”组＞ＨＢｅＡｇ（＋）组＞ＨＢｃＡｇ（＋）组＞ＨＢｓＡｇ（＋）＞“小三阳”和ＨＢｃＡｇ（＋）组＞“小三阳”组＞三项抗体有一项以上阳性组。结论：“大三阳”组的ＨＢＶＤＮＡ阳性率高于“小三阳”组，且同时伴ＨＢｃＡｇ（＋）时，ＨＢＶＤＮＡ的检出率较高。三项抗体有一项以上阳性组的ＨＢＶＤＮＡ阳性率较低，与其它各组比较，除“小三阳”组或伴ＨＢｃＡｇ（＋）组外，差异均有显著性（Ｐ＜０．０１），ＨＢＶＤＮＡ的检测已成为ＨＢＶ血清学标准的重要依据
Objective: To evaluate the clinical significance and superiority of polymerase chain reaction (PCR) in detection of serum HBV DNA. Methods: The HBV DNA of 81 serum HBV markers was detected by PCR and compared with the solid phase radioimmunoassay. Results: The positive rates of HBV DNA in serum were as follows: “Big Sanyang” and HBcAg (+) group> “Big Sanyang” group> HBeAg (+)> HBcAg (+)> HBsAg (+)> “And HBcAg (+) group>” small Sanyang “group> three antibodies have more than one positive group. Conclusion: The positive rate of HBV DNA in ”Sanyangyang“ group is higher than that in ”Sanyangyang“ group, and the detection rate of HBV-DNA is higher when HBcAg (+) is accompanied by the same time. Three antibodies had more than one positive group of HBV-DNA positive rate was lower, compared with other groups, except ”Sanyang" group or with HBcAg (+) group, the difference was significant (P <0.01) , HBV DNA detection has become an important basis for HBV serological standards