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为研究表皮生长因子(EGF)及表皮生长因子受体(EGFR)在急性放射性皮肤溃疡组织中的表达水平及对溃疡形成、发展、愈合的影响,采用雌性Wistar大鼠,以60Coγ射线局部照射法建立急性放射性皮肤溃疡动物模型,并以手术法建立单纯皮肤伤口动物模型,观察病变55d,采用免疫组化、原位杂交和图像分析等方法检测单纯伤口及皮肤溃疡组织中EGF及其受体的转录和表达水平。结果表明,照射后14d照射野内开始出现皮肤溃疡,之后逐渐扩大、融合、加深。皮肤受照射区多种细胞,特别是溃疡床表皮细胞、成纤维细胞及血管内皮细胞中EGF及其受体的转录及表达水平均较正常皮肤组织有所增强,但与单纯伤口组比较,溃疡组织中EGF及其受体的转录及表达水平明显降低。 说明辐射诱导的皮肤溃疡组织中EGF及其受体表达水平降低可能与溃疡发生、发展及难愈合的分子机制相关。
In order to study the expression of epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in acute radiation-induced skin ulcers and the effect on the formation, development and healing of ulcers, Wistar rats were used to investigate the effects of 60Co γ-ray irradiation Animal model of acute radiation-induced skin ulcer was established. Animal model of pure skin wound was established by surgery. The lesions were observed for 55 days. Immunohistochemistry, in situ hybridization and image analysis were used to detect the expression of EGF and its receptor Transcription and expression levels. The results showed that skin ulcers began to appear within 14 days after irradiation, then gradually expanded, fused and deepened. The transcription and expression of EGF and its receptor in many kinds of cells, especially in ulcerated bed epidermal cells, fibroblasts and vascular endothelial cells, were enhanced in skin irradiated area as compared with those in normal skin. However, Tissue EGF and its receptor transcription and expression levels were significantly reduced. These results indicate that the decrease of EGF and its receptor expression in skin ulcer induced by radiation may be related to the molecular mechanism of ulcer occurrence, development and refractory.