论文部分内容阅读
目的：以ＲＴ－ＰＣＲ技术测定和分析高脂高胆固醇膳食和有氧运动干预对高胆固醇血症大鼠肝脏卵磷脂胆固醇酯酰转移酶（ＬＣＡＴ）和载脂蛋白 ＡＩ（ａｐｏＡＩ）基因表达的影响，探讨有氧运动对胆固醇逆向转运（ＲＣＴ）通路影响的分子机理。方法：雄性ＳＤ大鼠４０只，随机分为：１）８周普通膳食对照组（ＮＳ，ｎ＝１０），２） ８周高脂膳食对照组（ＨＳ，ｎ＝１０），３）８周普通膳食＋运动组（ＮＥ，ｎ＝１０）和４）８周高脂膳食＋运动组（ＨＥ，ｎ＝１０）。建立高脂高胆固醇膳食诱导ＳＤ大鼠高脂血症的实验动物模型和有氧运动训练模型，用 ＲＴ－ＰＣＲ方法测定肝脏ＬＣＡＴ ｍＲＮＡ和ａｐｏＡＩ ｍＲＮＡ表达量。结果：（１）ＮＥ组大鼠肝脏ＬＣＡＴ ｍＲＮＡ的表达较ＮＳ组显著增高（Ｐ＜０．０５）；（２）ＨＳ组ＬＣＡＴ和ａｐｏＡＩ ｍＲＮＡ表达较ＮＳ组均显著降低（Ｐ＜０．０１）；（３）ＨＥ组ＬＣＡＴ和ａｐｏＡＩ ｍＲＮＡ较 ＨＳ组显著升高（Ｐ＜０．０１），且 ＬＣＡＴ ｍＲＮＡ与 ＮＳ组相比无显著差异，但是ａｐｏＡＩ ｍＲＮＡ的表达仍显著低于ＮＳ组（Ｐ＜０．０１）；（４）肝脏ａｐｏＡＩ ｍＲＮＡ的表达量与血清ＨＤＬ－Ｃ的水平无显著相关；在ＮＥ、ＨＳ和ＨＥ组中肝?
OBJECTIVE: To determine and analyze the effects of hypercholesterolemia diet and aerobic exercise on the gene expressions of lecithin cholesterol acyltransferase (LCAT) and apolipoprotein AI (apoAI) in hypercholesterolemic rats by RT-PCR To investigate the molecular mechanism of the effect of aerobic exercise on cholesterol reverse transport (RCT) pathway. Methods: Forty male Sprague-Dawley rats were randomly divided into 1) 8-week normal diet control group (NS, n = 10), 2) 8-week high fat diet control group (HS, n = 10), 3) Normal diet + exercise group (NE, n = 10) and 4) 8 week high fat diet + exercise group (HE, n = 10). The animal model of hyperlipidemia induced by high fat and cholesterol diet and aerobic exercise training model were established. The expressions of LCAT mRNA and apoAI mRNA in liver were measured by RT-PCR. Results: (1) The expression of LCAT mRNA in NE group was significantly higher than that in NS group (P <0.05). (2) The expression of LCAT and apoAI mRNA in HS group was significantly lower than that in NS group (P <0.01) ; (3) LCAT and apoAI mRNA in HE group were significantly higher than those in HS group (P <0.01), and there was no significant difference between LCAT mRNA and NS group, but the expression of apoAI mRNA in HE group was still significantly lower than that in NS group (P < 0.01). (4) There was no significant correlation between the expression of apoAI mRNA and the level of HDL-C in the liver.