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目的了解四川省部分地区温泉水中嗜肺军团菌污染状况,分析嗜肺军团菌SBT基因型别。方法随机采集省内部分温泉水进行嗜肺军团菌分离、鉴定和计数,对分离到的菌株进行血清分型、毒力基因(lvh、rtxA)检测、SBT基因分型。结果共采集水样27份,检出13株嗜肺军团菌,检出率为48.15%;分离到的嗜肺军团菌血清型包括Lp1、Lp3、Lp6、Lp9(或14)、Lp11和Lp13,以Lp1为主(占46.15%,6/13)。13株菌中,lvh、rtxA基因全部为阳性;13株嗜肺军团菌分为8种SBT型别,其中发现4种SBT新型。结论四川部分地区温泉水嗜肺军团菌污染比较严重,菌株均具有毒力,致病力强;温泉水中嗜肺军团菌SBT基因分型较分散,发现4个新的SBT型别。
Objective To understand the status of Legionella pneumophila contamination in hot spring water in some areas of Sichuan Province and analyze the genotypes of Legionella pneumophila SBT. Methods The clinical isolates of Legionella pneumophila were collected from some hot spring water in the province. Serotyping, virulence genes (lvh, rtxA) and SBT genotyping were performed on the isolated strains. Results A total of 27 samples of Legionella pneumophila were detected and the detection rate was 48.15%. The isolated L. pneumophila serotypes included Lp1, Lp3, Lp6, Lp9 (or 14), Lp11 and Lp13, Lp1 dominated (46.15%, 6/13). Of 13 strains, all lvh and rtxA genes were positive. Thirteen strains of Legionella pneumophila were divided into eight SBT types, of which four were found. Conclusion Legionella pneumophila polluted by hot spring water in some areas of Sichuan Province were seriously polluted. The strains were both virulent and virulent. The genotype of Legionella pneumophila SBT in hot spring water was scattered and four new SBT types were found.