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正常滋养层细胞的侵润受转化生长因子-β(TGF-β)的调控。该文研究了人正常细胞滋养层细胞(CTB)中TGF-β1对MMP-2和-9表达的调控。结果表明,TGF-β1抑制CTB细胞中MMP-9mRNA的表达和酶原MMP-9的分泌,但不影响MMP-2 mRNA和蛋白的表达。IL-1β和TGF-β1均能抑制MMP-9 mRNA的表达和酶原MMP-9的分泌,但二者的效应互相拮抗。抑制ERK1/2信号通路导致TGF-β1对MMP-9 mRNA和酶原MMP-9的抑制作用受阻。以上结果表明ERK1/2信号通路参与TGF-β31对人滋养层细胞MMP-9表达的抑制作用。
The invasion of normal trophoblastic cells is regulated by transforming growth factor-β (TGF-β). This article investigates the regulation of the expression of MMP-2 and -9 by TGF-β1 in human normal trophoblast cells (CTBs). The results showed that TGF-β1 inhibited the expression of MMP-9 mRNA and the secretion of proenzyme MMP-9 in CTB cells, but did not affect the expression of MMP-2 mRNA and protein. Both IL-1β and TGF-β1 could inhibit the expression of MMP-9 mRNA and the secretion of proenzyme MMP-9, but their effects antagonized each other. Inhibition of ERK1 / 2 signaling leads to inhibition of the inhibitory effect of TGF-β1 on MMP-9 mRNA and proenzyme MMP-9. These results indicate that ERK1 / 2 signaling pathway is involved in the inhibitory effect of TGF-β31 on human trophoblast MMP-9 expression.