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目的：探讨重组腺病毒介导反义 c-myc基因(Ad-ASmyc)治疗人肝癌细胞的作用。方法：观察 Ad-ASmyc对人肝癌细胞系的转导效率，通过细胞生长曲线、克隆形成实验、 DNA片段化分析、RT-PCR、裸鼠皮下移植瘤治疗实验，分析 Ad-ASmyc对人肝癌细胞系 Bel-7402、 QSG-7701、 SMMC-7721和 HCC-9204细胞生长和 c-myc基因表达及裸鼠肿瘤生长的抑制作用。结果： Ad-ASmyc可高效转导人肝癌细胞系，抑制细胞生长；转染细胞克隆形成能力降低，克隆成活率为对照组的53.9％～69.1％，c-myc基因表达下降；Ad-ASmyc处理肝癌细胞， DNA凝胶电泳出现明显的梯形条带，瘤内注射 Ad-ASmyc可抑制裸鼠皮下移植瘤生长。 结论：重组腺病毒介导的反义 c-myc基因转移，有可能成为肝癌基因治疗的一种有效方法。
Objective: To investigate the effect of recombinant adenovirus-mediated anti-sense c-myc gene (Ad-ASmyc) on human hepatoma cells. Methods: The transduction efficiency of Ad-ASmyc on human hepatocellular carcinoma cell line was observed. Cell proliferation curve, clone formation assay, DNA fragmentation analysis, RT-PCR, and nude mice subcutaneous transplantation tumor treatment experiment were used to analyze Ad-ASmyc on human hepatoma cells. It is the inhibition of Bel-7402, QSG-7701, SMMC-7721 and HCC-9204 cell growth and c-myc gene expression and tumor growth in nude mice. Results: Ad-ASmyc could efficiently transduce human hepatocellular carcinoma cell lines and inhibit cell growth. The colony formation ability of transfected cells was decreased. The clone survival rate was 53.9%-69.1% of the control group, and c-myc gene expression was decreased. Ad-ASmyc treatment In liver cancer cells, DNA ladders showed obvious trapezoidal bands. Intratumoral injection of Ad-ASmyc inhibited the growth of subcutaneous transplanted tumors in nude mice. Conclusion: The recombinant adenovirus-mediated antisense c-myc gene transfer may be an effective method for gene therapy of liver cancer.