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目的探讨广东汉族妇女解毒酶细胞色素P450 1A1(CYP1A1)基因 MspⅠ多态性与子宫内膜异位症(内异症)的遗传易感性的关系。方法用聚合酶链反应-限制性片段长度多态性技术,检测76例经腹腔镜或手术证实为内异症的患者(内异症组)和80例非内异症的妇科手术患者(对照组)CYP1A1基因3’端限制性内切酶MspⅠ位点的3种基因型(m1m1、m1m2、m2m2)的分布频率并加以分析,2组检测对象均为广东籍汉族妇女。结果CYP1A1基因 MspⅠ位点基因型m2m2在内异症组和对照组中各占19.7%和12.5%,基因型m1m1在2组中分别占30.3%和42.5%,基因型m1m2在2组中分别占50.0%和45.0%,各基因型在2组中所占比例无显著性差异(P>0.05);m1、m2等位基因频率2组间比较亦无显著性差异(P>0.05)。结论解毒酶CYP1A1基因MspⅠ多态性的单独存在可能与广东籍汉族妇女内异症的易感性无关。
Objective To investigate the relationship between Msp Ⅰ polymorphism of cytochrome P450 1A1 (CYP1A1) gene and the susceptibility of endometriosis (endometriosis) in Guangdong Han women. Methods The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used in 76 patients with endometriosis confirmed by endoscopy or surgery (endometriosis group) and 80 patients with non-endometriosis gynecological surgery Group). The frequencies of the three genotypes (m1m1, m1m2, m2m2) of the 3 ’end restriction endonuclease MspⅠ site of CYP1A1 gene were analyzed and analyzed. All subjects in the two groups were Han women of Guangdong. Results The genotypes of mspⅠgene in the CYP1A1 gene, m2m2, were 19.7% and 12.5% in the endometriosis group and control group, respectively. The genotype m1m1 accounted for 30.3% and 42.5% of the two groups respectively. The genotype m1m2 accounted for 50.0% and 45.0%, respectively. There was no significant difference in the proportion of genotypes among the two groups (P> 0.05). There was no significant difference between the two groups in allele frequencies of m1 and m2 (P> 0.05). Conclusion The existence of the Msp Ⅰ polymorphism of CYP1A1 gene in detoxification enzyme may not be related to the susceptibility of Han women in Guangdong.