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１引言根据染料与蛋白质的相互作用建立测定蛋白质的分析方法已有不少报道，但寻找简便、快速、准确的测定蛋白质的分析方法仍然是人们研究的热点。生物大分子与小分子配体相互作用的研究，目前多用透析，超滤或凝胶色谱等实验方法。当小分子配体与生物大分子结合前后的吸收光?
1 Introduction According to the dye-protein interaction assay to establish the determination of protein has been reported, but to find a simple, rapid and accurate method for the determination of protein analysis is still the focus of research. Biological macromolecules and small molecule ligand interaction studies, the current multi-purpose dialysis, ultrafiltration or gel chromatography and other experimental methods. When the small molecule ligand and biological macromolecules before and after the absorption of light?