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目的探讨DKK-1在食管癌患者血清中表达的临床意义。方法应用酶联免疫(Elisa)法检测80例食管鳞癌患者和35例正常人血清中的DKK-1的表达水平,并与常规肿瘤标记物进行比较,探讨其在食管癌中的诊断价值。结果食管鳞癌患者血清中DKK-1浓度为(90.44±57.41)ng/mL,而正常人血清浓度为(11.29±9.45)ng/mL,两者之间差异有统计学意义(P<0.01)。若以正常人血清DKK-1浓度95%可信区间上限为界值(14.54 ng/mL),则食管癌鳞患者血清中DKK-1的敏感性为66.25%(53/80),特异性为82.86%;血清中的DKK-1的阳性率仅为17.14%(6/35),两者之间差异有统计学意义(P<0.01)。经过统计学分析显示,食管鳞癌患者血清中的DKK-1水平与肿瘤大小、分期及淋巴结转移等因素相关(P<0.05),而与性别、年龄及肿瘤的分化程度无关(P>0.05)。通过与其他标记物比较发现DKK-1的检测对食管鳞癌诊断的敏感度明显高于常规肿瘤标记物(CEA 39.44%,CY-FRA21-1 25.35%,P<0.01),三者联合检测能明显提高诊断食管癌的敏感性。结论DKK-1可作为检测食管癌的一种新的肿瘤标记物,对食管癌的诊断和病情评估有一定的辅助参考价值。
Objective To investigate the clinical significance of DKK-1 expression in the serum of esophageal cancer patients. Methods The serum levels of DKK-1 in 80 cases of esophageal squamous cell carcinoma and 35 cases of normal human esophageal squamous cell carcinoma were detected by enzyme-linked immunosorbent assay (ELISA), and compared with conventional tumor markers. Results The serum level of DKK-1 in esophageal squamous cell carcinoma was (90.44 ± 57.41) ng / mL, while the normal serum was (11.29 ± 9.45) ng / mL, with significant difference between the two groups (P <0.01) . The sensitivity of DKK-1 in serum of esophageal squamous cell carcinoma was 66.25% (53/80) with the specificity of 95% confidence interval of serum DKK-1 as the cutoff (14.54 ng / mL) 82.86% respectively; The positive rate of DKK-1 in serum was only 17.14% (6/35), the difference was statistically significant (P <0.01). After statistical analysis, the serum level of DKK-1 in patients with esophageal squamous cell carcinoma correlated with tumor size, stage and lymph node metastasis (P <0.05), but not with sex, age and tumor differentiation (P> 0.05) . Compared with other markers, the sensitivity of DKK-1 to the diagnosis of esophageal squamous cell carcinoma was significantly higher than that of routine tumor markers (CEA 39.44%, CY-FRA21-1 25.35%, P <0.01) Significantly improve the diagnosis of esophageal cancer sensitivity. Conclusions DKK-1 can be used as a new tumor marker in the detection of esophageal cancer, which may be helpful for the diagnosis and assessment of esophageal cancer.