用棉塞作为菌种瓶的封口不仅成本高,灭菌时还常润湿棉塞,容易导致菌种污染。下面介绍几种不用棉塞的菌种瓶封口法: 1.用四层纱布外加一层牛皮纸蒙口,用牛筋扎紧。 2.用双层报纸外加一层牛皮纸蒙口,用牛筋扎紧。 3.用双层牛皮纸蒙口,用牛筋扎紧。 4.用双层报纸外加一层聚丙烯薄膜,用牛筋扎紧。灭菌接种后,薄膜只松松地扎在报纸层外面,以增加透气性。 5.用一层聚丙烯薄膜并用牛筋扎口。此法仅用于耐受较高浓度二氧化碳的平菇、凤尾菇菌种,且不宜太长时间存放菌种,以 Tapping the tampon as a kind of strain bottle not only has high cost, but also often moistens the tampon when sterilizing, which can easily cause strain contamination. The following describes several kinds of bacteria without tampons bottle sealing method: 1. With a layer of four layers of gauze plus a layer of kraft paper covered with beef tendon tie tight. 2. With a layer of newspaper plus a layer of kraft paper Meng mouth, tied with tendon. 3. Double-coated with kraft paper, tied with tendon. 4. With a double layer of polypropylene plus a layer of film, tied with tendon. After inoculation, the film is loosely attached to the outside of the newspaper layer to increase breathability. 5. Use a layer of polypropylene film and tie with tendon. This method is only used to tolerate the higher concentration of carbon dioxide Pleurotus ostreatus mushroom strains, and should not be too long to store bacteria to