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目的:了解厦门地区婴幼儿腹泻诺如病毒(NV)分子流行病学特征。方法:收集2010年5月～2011年4月全年轮状病毒抗原阴性的急性非细菌性腹泻标本,用实时荧光RT-PCR法扩增NV ORF1-ORF2结合区,测序扩增片段后进行比对、分型及构建系统进化树。结果:全年共获得366份标本,共检测出81份为NV阳性标本,其中GI组5例,GII组76例;NV在厦门地区全年可见散发,但在每年7和11月有明显的流行高峰,且腹泻感染患儿主要集中在3岁以下;已分型的NV基因组(55例)以GII-4/2006b占绝对优势,其构成比达60.00%(33/55),其余为GII-3占25.45%(14/55),GII-6占5.45%(3/55),GII-2占3.64%(2/55),此外GII-12、GI-4、GI-5各占1.81%(1/55)。结论:厦门地区NV夏秋季发病率较高,GII-4/2006b变异株为优势株,且感染的NV可能存在病毒重组。
Objective: To understand the molecular epidemiology of infantile diarrhea Norovirus (NV) in Xiamen. METHODS: Acute non-bacterial diarrhea specimens were collected from May 2010 to April 2011 for the whole year. Real-time fluorescent RT-PCR was used to amplify the region of NV ORF1-ORF2 binding. The amplified fragments were sequenced and compared Pair, type, and construct phylogenetic trees. Results: A total of 366 specimens were obtained in the year, of which 81 were detected as NV positive specimens, including 5 cases in GI group and 76 cases in GII group. NV was found throughout the year in Xiamen, but significant in July and November Prevalence peak, and children with diarrhea infection mainly concentrated in the age of 3 years; genotyped NV genome (55 cases) to GII-4 / 2006b accounted for absolute advantage, the constituent ratio of up to 60.00% (33/55), the rest of GII -3 accounted for 25.45% (14/55), GII-6 accounted for 5.45% (3/55), GII-2 accounted for 3.64% (2/55), in addition to GII-12, GI-4 and GI- % (1/55). Conclusion: The incidence of NV in Xiamen is high in summer and autumn, and the GII-4 / 2006b mutant is the predominant strain, and the infected NV may have virus recombination.