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目的研究铅对大鼠中枢神经元的损伤以及应用维生素C后的影响,探讨维生素C对铅中毒条件下中枢神经元的保护作用。方法将健康1周龄Wistar大鼠随机分为铅染毒组,维生素C给药组及生理盐水组。各组于出生后第2周、第3周、第4周取材。铅染毒组、维生素C给药组每组18只,生理盐水组每组6只,共42只。测量血铅,分别进行凋亡抑制基因Bcl-2、凋亡基因Bax免疫组织化学染色及Fluoro-Jade B荧光染色,并作光密度分析。结果通过对各个时间段各组血铅测定,可得维生素C给药组血铅含量为(2.395±0.974)μmol/L,低于铅染毒组(3.164±1.481)μmol/L。Bcl-2铅染毒组阳性细胞表达少于给药组(P<0.01),而Bax多于给药组(P<0.01),Flouro-Jade B铅染毒组阳性细胞率明显多于给药组(P<0.01)。结论维生素C可降低染铅大鼠的血铅浓度,并具有一定抗细胞损伤作用,对染铅大鼠的中枢神经元具有一定的保护作用。
Objective To study the effects of lead on central neurons in rats and the effects of vitamin C on the protection of central neurons under lead poisoning. Methods Healthy Wistar rats of 1 week old were randomly divided into lead poisoning group, vitamin C group and saline group. Groups were drawn at 2 weeks, 3 weeks and 4 weeks after birth. Lead poisoning group, vitamin C treatment group, each group of 18, saline group of 6, a total of 42. Blood lead levels were measured. Bcl-2, Bcl-2 and Fluoro-Jade B were detected respectively. The optical density was analyzed. Results The blood lead level in each vitamin C group was (2.395 ± 0.974) μmol / L, which was lower than that in the lead group (3.164 ± 1.481) μmol / L. The positive cells of Bcl-2 lead-exposed group were less than that of the drug-treated group (P <0.01), while Bax was more than that of the drug-treated group (P <0.01) Group (P <0.01). Conclusion Vitamin C can reduce blood lead levels in lead-exposed rats and have a certain anti-cell injury effect, which can protect central nervous system of lead-exposed rats.