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研究了环境盐度急性胁迫对鲻鱼幼鱼(Mugil cephalus)鳃丝Na+/K+-ATPase(NKA)活性及体内含水量的影响。结果表明,将幼鱼从S33(盐度33)的对照组中直接转移至S0(盐度0)、S10(盐度10)、S20(盐度20)、S33和S40(盐度40)的水体中,随盐度降低各盐度处理的死亡率迅速升高。各处理组鱼的鳃丝NKA活性在最初3 d内出现一定波动,随后变化平稳。实验结束时(12 d),NKA活性随盐度梯度呈“∩”型分布,最终(12 d时),S10、S20处理组酶活性显著高于其他处理组(P<0.05),S33处理组活性最低。不同盐度的去内脏组和全鱼组含水量比较结果均为S0>S20>S10>S33>S40。从总体结果来看,鲻鱼幼鱼在9 d左右适应不同盐度的水环境。在稳定期,全鱼组(12 d)与去内脏组(14 d)比较,除S0处理组的鲻鱼含水量去内脏组大于全鱼组,其余各组都是全鱼组大于去内脏组。
The effects of acute environmental salinity stress on Na + / K + -ATPase (NKA) activity and water content in gill of Mugil cephalus were studied. The results showed that juveniles were directly transferred from the control group of S33 (Salinity 33) to S0 (Salinity 0), S10 (Salinity 10), S20 (Salinity 20), S33 and S40 (Salinity 40) In the water body, the salinity treatment mortality increases rapidly with decreasing salinity. The NKA activity of gill filaments in each treatment group fluctuated within the first 3 days and then changed smoothly. At the end of the experiment (12 d), the activity of NKA was distributed along with the salinity gradient. At the end of 12 d, the enzyme activities in S10 and S20 treatments were significantly higher than those in other treatments (P <0.05) The treatment group had the lowest activity. The results of water content of the viscera group and the whole fish group at different salinities were S0> S20> S10> S33> S40. From the overall results, juvenile catfish adapt to different salinity water environment around 9 days. In the stationary phase, the whole fish group (12 d) compared with the eviscerated group (14 d), except for the S0 group, the water content of anchovy in the visceral group was greater than that in the whole fish group, .