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目的观察红花注射液(ICT)对2,4,6-三硝基苯磺酸(2,4,6-TNBS)所致大鼠溃疡性结肠炎(UC)的疗效及其对结肠组织中EGF和TGF-β1表达的影响。方法将大鼠随机分为3组:模型组、ICT组和正常对照组(n均为10)。用TNBS灌肠复制大鼠UC模型,用ICT干预治疗,观察大鼠疾病活动指数(DAI)及大肠组织学损伤以判断疗效;用免疫组化法检测UC大鼠结肠黏膜EGF和TGF-β1的表达,用逆转录-聚合酶链反应(RT-PCR)法检测EGF mRNA和TGF-β1mRNA的表达,从而探讨其治疗机制。结果与模型组相比,ICT组的DAI及组织学损伤显著改善(P<0.05);与正常对照组相比,ICT组与模型组大鼠结肠组织中EGF,TGF-β1蛋白及其mRNA的表达均显著增高(P<0.05);而与模型组相比,ICT组结肠组织中EGF及其mRNA的表达均显著增高(P<0.05);TGF-β1蛋白及其mRNA的表达显著降低(P<0.05)。结论 ICT对TNBS所致大鼠UC具有一定疗效,ICT可以增加EGF,TGF-β1的表达,促进创伤组织的修复,是ICT治疗溃疡性结肠炎可能的作用机制之
Objective To observe the curative effect of safflower injection (ICT) on ulcerative colitis (UC) induced by 2,4,6-trinitrobenzene sulfonic acid (2,4,6-TNBS) in rats and its effect on colonic tissue EGF and TGF-β1 expression. Methods The rats were randomly divided into 3 groups: model group, ICT group and normal control group (n = 10). UC rats were treated with TNBS and the intervention of ICT was used to evaluate the disease activity index (DAI) and colorectal mucosal injury in rats. The expressions of EGF and TGF-β1 in colonic mucosa of UC rats were detected by immunohistochemistry The expression of EGF mRNA and TGF-β1 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR), and the therapeutic mechanism was explored. Results Compared with the model group, the DAI and histological damage in ICT group were significantly improved (P <0.05). Compared with the normal control group, the expression of EGF, TGF-β1 protein and mRNA in the colon tissue of ICT group and model group (P <0.05). Compared with the model group, the expression of EGF and its mRNA in the colon of ICT group was significantly increased (P <0.05), while the expression of TGF-β1 protein and mRNA was significantly decreased (P <0.05). Conclusions ICT has a certain curative effect on TNBS-induced UC in rats. ICT can increase the expression of EGF and TGF-β1 and promote the repair of wound tissue, which may be the possible mechanism of ICT for the treatment of ulcerative colitis