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血中癌胚抗原(CEA)虽作为肿瘤标志物而被广泛应用,但组织内CEA及临床意义报告甚少。作者通过测定血液与组织的CEA,探讨癌组织CEA的产生与癌肿进展关系以及临床意义。方法:83例结肠癌病人(Dukes分类为A、BC的各有7、27、49例。C中淋巴结转移14例、腹膜转移35例。)组织CEA提取,在TEMG缓冲液中匀浆3次,离心(105,000×g)合并上清液。组织与血清提取液经煮沸后再离心,上清液用RIA法测定。
Although carcinoembryonic antigen (CEA) in blood is widely used as a tumor marker, there are few reports of CEA in tissue and its clinical significance. By measuring the CEA in blood and tissues, the authors explored the relationship between the occurrence of cancerous CEA and the progression of cancer and its clinical significance. Methods: 83 patients with colon cancer (Dukes were classified as A, BC, respectively, 7, 27, 49 cases. C lymph node metastasis in 14 cases, peritoneal metastasis in 35 cases.) Tissue CEA extraction, homogenized in TEMG buffer 3 times The supernatant was combined by centrifugation (105,000 x g). Tissue and serum extracts were centrifuged after centrifugation and supernatants were assayed by RIA.