In order to investigate the physiological effects of airport noise exposure on organisms,in this study,we exposed Sprague-Dawley rats in soundproof chambers to previously recorded aircraft-related noise for 65 d.For comparison,we also used unexposed control rats.Noise was arranged according to aircraft flight schedules and was adjusted to its weighted equivalent continuous perceived noise levels(LWECPN) of 75 and 80 dB for the two experimental groups.We examined rat behaviors through an open field test and measured the concentrations of plasma norepinephrine(NE) by high performance liquid chromatography-fluorimetric detection(HPLC-FLD).We also examined the morphologies of neurons and synapses in the temporal lobe by transmission electron microscopy(TEM).Our results showed that rats exposed to airport noise of 80 dB had significantly lower line crossing number(P<0.05) and significantly longer center area duration(P<0.05) than control animals.After 29 d of airport noise exposure,the concentration of plasma NE of exposed rats was significantly higher than that of the control group(P<0.05).We also determined that the neuron and synapsis of the temporal lobe of rats showed signs of damage after aircraft noise of 80 dB exposure for 65 d.In conclusion,exposing rats to long-term aircraft noise affects their behaviors,plasma NE levels,and cell morphology of the temporal lobe. In order to investigate the physiological effects of airport noise exposure on organisms, in this study, we exposed Sprague-Dawley rats in soundproof chambers to previously recorded aircraft-related noise for 65 d. For comparison, we also used unexposed control rats. No. was was arranged according to aircraft flight schedules and was adjusted to its weighted equivalent continuous perceived noise levels (LWECPN) of 75 and 80 dB for the two experimental groups. We examined rat behaviors through an open field test and measured the concentrations of plasma norepinephrine (NE) by high performance liquid chromatography-fluorimetric detection (HPLC-FLD). We also examined the morphologies of neurons and synapses in the temporal lobe by transmission electron microscopy (TEM) .Our results showed that rats that are exposed to airport noise of 80 dB had significantly lower line crossing number (P <0.05) and significantly longer center area duration (P <0.05) than control animals.After 29 d of airport noise exposure, the conc entration of plasma NE of exposed rats was significantly higher than that of the control group (P <0.05) .We also determined that the neuron and synapsis of the temporal lobe of rats showed signs of damage after aircraft noise of 80 dB exposure for 65 d In conclusion, exposing rats to long-term aircraft noise affects their behaviors, plasma NE levels, and cell morphology of the temporal lobe.