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目的确定95%灭菌丹原药对SD大鼠的毒性作用,并探寻其可能的毒性靶器官。方法将80只SD大鼠随机分成4组,各组灭菌丹剂量为0、4 000、8 000及16 000 mg/kg饲料。试验结束后检测动物血常规及生化指标,对解剖动物脏器进行组织病理学评价。结果试验期间,中、高剂量灭菌丹组大鼠体重均明显降低(P<0.05)。与对照组比较,中、高剂量灭菌丹组大鼠血液中性粒细胞百分率升高,差异均有统计学意义(P<0.05);雄鼠中、高剂量组脑、心脏、脾脏、肝脏、肾脏及肾上腺的绝对重量均高于对照组(P<0.05);中、高剂量灭菌丹组大鼠前胃粘膜下水肿及炎性细胞浸润的发生率均明显高于对照组(P<0.05)。结论在本试验条件下,前胃是95%灭菌丹潜在的毒性靶器官,该农药的最大无作用剂量为4 000 mg/kg饲料。
Objective To determine the toxic effect of 95% sterilized Dan original drug on SD rats and explore its possible toxic target organ. Methods Eighty SD rats were randomly divided into 4 groups. The dosages of sterilized Dan in each group were 0, 4 000, 8 000 and 16 000 mg / kg. After the test, blood routine and biochemical indexes of animals were tested, and histopathological evaluation of anatomical animal organs was conducted. Results During the experiment, the body weight of middle and high dose of Danzhutan group was significantly decreased (P <0.05). Compared with the control group, the percentage of blood neutrophils in middle and high dosage of Danzhutan group was significantly increased (P <0.05); in the medium and high dosage Danzhutan group, the percentage of neutrophil in the blood, brain, heart, spleen and liver (P <0.05). The incidences of anterior gastric submucosal edema and inflammatory cell infiltration in middle and high dosage of Danzhitongdan group were significantly higher than those in the control group (P < 0.05). Conclusion Under the test conditions, the anterior stomach is a potential toxic target organ of 95% bacteriocin. The maximum no dose of this pesticide is 4 000 mg / kg feed.