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探讨干扰素治疗慢性乙型肝炎 (CHB)患者对外周血树突状细胞 (DCs)分泌IL - 12的影响。采集并分离2 3例干扰素治疗前、后CHB患者的外周血单个核细胞 ,在rhIL - 4和rhGM -CSF的作用下培养 10天 ,收集上清液检测IL - 12的水平 ,以 8例健康人作为对照。干扰素治疗前患者DCs分泌IL - 12的水平显著低于对照组 (P <0 0 1) ,治疗 4个月时DCs的分泌水平较治疗前显著增加 (P <0 0 5 )。进一步分析发现 ,治疗后IL - 12的水平仅在完全应答组较治疗前显著性增加 (P <0 0 1)。提示CHBDCs分泌IL - 12的水平低下 ,干扰素治疗可以促进DCs产生IL -12 ,且其增加的程度与干扰素的疗效有关
To investigate the effect of interferon on the secretion of IL - 12 by peripheral blood dendritic cells (DCs) in patients with chronic hepatitis B (CHB). Peripheral blood mononuclear cells from CHB patients before and after treatment with 23 interferons were collected and isolated. The cells were cultured with rhIL - 4 and rhGM - CSF for 10 days. The supernatants were collected to detect the levels of IL - 12. Eight patients Healthy people as a control. The levels of IL - 12 secreted by DCs in patients with interferon before treatment were significantly lower than those in the control group (P <0.01). The secretions of DCs at 4 months after treatment were significantly increased (P <0.05). Further analysis showed that the level of IL - 12 increased only significantly after treatment in the complete response group compared with that before treatment (P <0.01). It is suggested that the levels of IL - 12 secreted by CHBDCs are low, and the interferon treatment can promote the production of IL - 12 in DCs, and the extent of its increase is related to the efficacy of interferon