论文部分内容阅读
目的:观察(R, R)-XY-10和(S, S)-XY-10对视网膜色素上皮细胞的增生作用,并且进一步的研究其作用机制。但(R, R)-XY-10和(S, S)-XY-10对人脐静脉内皮细胞并无增生的作用。方法:通过人视网膜色素上皮细胞(ARPE-19)和人脐静脉内皮细胞(HUVECs)研究(R, R)-XY-10和(S, S)-XY-10对视网膜色素上皮细胞的增生作用,并且采用ERK、KT、PI3K、蛋白激酶C(PKC)和一氧化氮合酶(NOS)抑制剂来研究其作用机制。结果:(R, R)-XY-10和(S, S)-XY-10促进了ARPE-19细胞的增生,并具有剂量依赖性,但是对HUVECs细胞没有影响。如果同时加入增生抑制剂H7 (5μmol/L)、金丝桃素(20μmol/L)、PD98059(2μmol/L)、LY294002(50μmol/L)、SH-5(10μmol/L)和L-NAME (100μmol/L),则给予H7、金丝桃素、PD98059和LY294002各组的增生作用受到了抑制,而给予SH-5和L-NAME两组的增生作用没有影响。结论:(R, R)-XY-10和(S, S)-XY-10能够诱导ARPE-19细胞增生,其作用可能是通过MAPK和PI3K的途径来发挥该作用。因此,(R, R)-XY-10和(S, S)-XY-10能通过修复损伤的RPE细胞来治疗老年性黄斑变性。
OBJECTIVE: To observe the proliferative effects of (R, R) -XY-10 and (S, S) -XY-10 on retinal pigment epithelial cells and to further investigate its mechanism of action. However, (R, R) -XY-10 and (S, S) -XY-10 had no proliferative effects on human umbilical vein endothelial cells. Methods: The proliferative effects of (R, R) -XY-10 and (S, S) -XY-10 on retinal pigment epithelial cells were studied by human retinal pigment epithelial cells (ARPE-19) and human umbilical vein endothelial cells (HUVECs) , And the mechanism of action was investigated using inhibitors of ERK, KT, PI3K, protein kinase C (PKC) and nitric oxide synthase (NOS). Results: (R, R) -XY-10 and (S, S) -XY-10 promoted the proliferation of ARPE-19 cells in a dose-dependent manner but had no effect on HUVECs. If hyperinhibitor H7 (5μmol / L), hypericin (20μmol / L), PD98059 (2μmol / L), LY294002 (50μmol / L), SH-5 (10μmol / L) and L-NAME 100μmol / L), the hyperplastic effects of H7, hypericin, PD98059 and LY294002 were inhibited. However, the proliferation of SH-5 and L-NAME groups was not affected. CONCLUSION: (R, R) -XY-10 and (S, S) -XY-10 can induce the proliferation of ARPE-19 cells, which may play a role through MAPK and PI3K pathways. Therefore, (R, R) -XY-10 and (S, S) -XY-10 can treat age-related macular degeneration by repairing damaged RPE cells.