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大豆的产量构成因素中每荚粒数是提高大豆产量的育种关键因素,因此研究与荚粒数相关的基因至关重要,花芽作为植物生殖生长的第一步,对大豆植株的生长发育有着重要的影响,可能对四粒荚的产生有影响。以大豆吉农18四粒荚突变体的7叶期花芽的转录组测序结果进行分析,筛选出差异表达的片段Gm FBDG01;利用RT-PCR克隆差异表达基因的CDS核心序列,并借助中间克隆载体p Bluescript SK plus,成功构建了RNA干扰(RNAi)表达载体p CPB-Gm FBDG01-RNAi,该表达载体的构建为大豆四粒荚相关功能基因的功能验证奠定基础。
Therefore, it is very important to study the genes related to the number of pods and grain. As the first step of plant reproductive growth, flower buds are important to the growth and development of soybean plants The impact may have an impact on the production of four pods. The Gm FBDG01 fragment was screened from the transcriptome of 7-leaf stage flower buds of four pod mutants of soybean, Ji-Nong 18. The CDS core sequence of the differentially expressed genes was cloned by RT-PCR and cloned by means of intermediate cloning vector p Bluescript SK plus, the RNA interference (RNAi) expression vector pCPB-Gm FBDG01-RNAi was successfully constructed. The construction of this expression vector lays a foundation for the functional verification of four pod related genes.