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以“中黄13”大豆为材料,研究处理液浓度分别为100,200,300,500mg/L的阿魏酸和丁香酸对大豆种子萌发及幼苗生长的影响。结果表明,2种化感物质不同浓度处理液对大豆种子萌发及幼苗生长均能够产生显著的化感效应。4种浓度的阿魏酸处理液对大豆种子萌发均表现出抑制作用。浓度为500mg/L的阿魏酸抑制效果最为显著。较低浓度(100,200,300mg/L)的丁香酸处理液对大豆种子发芽率未表现出明显的影响,却推迟了种子萌发,显著降低了种子的发芽势和发芽指数。浓度为500mg/L的丁香酸显著抑制了大豆种子萌发。阿魏酸和丁香酸对大豆幼苗鲜重、根长、株高具有抑制作用,且抑制作用随处理浓度的增加而增强。4种处理浓度的阿魏酸和丁香酸均使大豆幼苗中的MDA含量增加,叶绿素含量降低,使大豆幼苗下表皮气孔长度减小,打开的气孔个数减少,气孔数目增多。
The effects of ferulic acid and syringic acid at 100, 200, 300 and 500 mg / L on seed germination and seedling growth of soybean were studied using “Zhonghuang 13” soybean as material. The results showed that the two allelochemicals with different concentrations of the treatment solution on soybean seed germination and seedling growth can produce a significant allelopathic effect. Four concentrations of ferulic acid treatment of soybean seed germination were inhibited. The concentration of 500mg / L of ferulic acid inhibition of the most significant. Syringobacteria treatment solutions at lower concentrations (100, 200, 300 mg / L) did not show obvious effect on the germination rate of soybean seeds, but delayed the seed germination and significantly reduced the germination potential and germination index of the seeds. Syringic acid at a concentration of 500 mg / L significantly inhibited soybean seed germination. Ferulic acid and syringic acid inhibited the fresh weight, root length and plant height of soybean seedlings, and the inhibitory effect increased with the increase of the treated concentration. Four treatments of ferulic acid and syringic acid all increased MDA content and decreased chlorophyll content in soybean seedling so that the stomatal length decreased, the number of stomata opened and the number of stomata increased.